Optical properties

  • λ abs = 343 nm
  • ε max = 1.9×104 M -1 cm -1
  • λ fl = 441 nm
  • n fl = 90 %
  • τ fl = 4.8 ns
  • CF 260 = ε 260 max = 0.19
  • CF 280 = ε 280 / ε max = 0.15

ATTO 343H is a hydrophilic short wavelength fluorescent marker with a coumarin structure. The characteristic properties of this dye include high fluorescence quantum yield, large Stokes-shift, good photostability and low molecular weight. ATTO 343H s very water soluble and the fluorescence efficiency after coupling to biomolecules, such as a protein, is only slightly reduced even at higher degrees of labeling (DOL). ATTO 343H is an anionic dye. After conjugation to a biomolecule, the dye carries a single negative charge. Efficient excitation of fluorescence occurs in the 330 - 365 nm range.

ATTO 343H

ATTO 343H is a hydrophilic short wavelength fluorescent marker with a coumarin structure. The characteristic properties of this dye include high fluorescence quantum yield, large Stokes-shift, good photostability and low molecular weight.

Optical properties

  • λ abs = 343 nm
  • ε max = 1.9×104 M -1 cm -1
  • λ fl = 441 nm
  • n fl = 90 %
  • τ fl = 4.8 ns
  • CF 260 = ε 260max = 0.19
  • CF 280 = ε 280 / ε max = 0.18

ATTO 343H is a hydrophilic short wavelength fluorescent marker with a coumarin structure. The characteristic properties of this dye include high fluorescence quantum yield, large Stokes-shift, good photostability and low molecular weight. ATTO 343H is very water soluble and the fluorescence efficiency after coupling to biomolecules, such as a protein, is only slightly reduced even at higher degrees of labeling (DOL). ATTO 343H is an anionic dye. After conjugation to a biomolecule, the dye carries a single negative charge. Efficient excitation of fluorescence occurs in the 330 - 365 nm range.

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

Fields of Application

Life Science Research

Leica Microsystems’ life science research microscopes support the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and…
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