ATTO 553H

ATTO 553H is a fluorescent marker from the rhodamine class.

Optical properties

• λ _abs = 553 nm
• ε _max = 1.2×10⁵ M ^-1 cm ^-1
• λ _fl = 575 nm
• n _fl = 85%
• τ _fl = 3.6 ns
• CF ₂₆₀ = ε ₂₆₀ /ε _max = 0.27
• CF ₂₈₀ = ε ₂₈₀ / ε _max = 0.12

ATTO 553H is a fluorescent marker from the rhodamine class. As a hydrophilic alternative to ATTO 550, the dye exhibits excellent water solubility and a significantly reduced tendency toward nonspecific binding and aggregation. Like ATTO 550, ATTO 553H also exhibits strong absorption, very high fluorescence quantum yield, and high thermal and photochemical stability. The dye is therefore ideal for use in single-molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 553H is an anionic dye. After coupling to a substrate, it is singly negatively charged. In contrast to ATTO 550 , ATTO 553H is present in isomerically pure form. Efficient fluorescence excitation occurs in the 540–565 nm range.

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems