THUNDER Imager Live Cell & 3D Assay
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THUNDER Imager Live Cell & 3D Assay
Decode 3D biology in real time*
The following configurations were used in the applications provided in the examples.
THUNDER Imagers can be configured to your application. Get in contact with us.
| THUNDER Imager | |||
| 3D Assay | Live Cell | Live Cell | |
| Specimen | Retina | Brain organoid | Explant aorta |
| Imaging Method | THUNDER | THUNDER | THUNDER |
| Workflow | Navigator spiral scan | Navigator large tile scan | Long-term live cell culture |
| Stand | DMi8 | DMi8 | DMi8 |
| Focus | Z-Focus, motorized, closed-loop | THUNDER AFC (hardware focus for fast focusing and drift correction) | Motorized, closed-loop, AFC (hardware focus for fast focusing and drift correction) |
| Stage | Scanning stage | QUANTUM high speed stage (real-time controlled with Synapse) | QUANTUM high speed stage (real-time controlled with Synapse) |
| Transmitted light contrast & method | BF, DIC | BF | BF |
| EFW | - | + | + |
| Filter cubes | Single band pass | Quad | Quad |
| Fluorescence illumination | LED3 | LED5 | LED8 |
| Incubation | - | Stage-top incubation | Incubator i8, Temp, CO2 control |
| Detection | K5 | DFC9000 GTC | DFC9000 GTC, Hamamatsu Flash4 |
| Optional Upgrade path for advanced imaging workflows | - | + | + |
| Optional Photomanipulation | - | Infinity Scanner | Infinity Scanner |
| Optional TIRF | - | TIRF | TIRF |
Core strengths of our THUNDER Imagers:
| THUNDER Imager | |||
| Applications | 3D Assay | Live Cell | |
| Monocell layers | ++ | +++ | |
| Organoids or Spheroids | ++ | +++ | |
| Zebrafish or C.Elegans, and other model organisms | ++ | +++ | |
| Whole organs i.e. bio-printed or uncleared tissue of Mos | ++ | +++ | |
| Thick tissue sections, i.e. biopsies or vibratome sections | ++ | +++ | |
| Carriers | |||
| Multi-well plates | +++ | +++ | |
| Chamber slides | ++ | +++ | |
| Glass slides | ++ | +++ | |
| Petri dishes | + | +++ | |
| Techniques | |||
| Live cell imaging (Time lapse) | + | +++ | |
| Contrast Methods (PhaCo, DIC, IMC) | +++ | ++ | |
| TIRF | - | +++ | |
| Photomanipulation | - | +++ | |
| Ablation, i.e. FRAP/FRET | - | +++ | |