Guenter Resch , Dr.
Dr. Guenter Resch is a cell biologist by training with many years of experience in electron microscopy. He is owner and founder of Nexperion, an independent company located in Vienna/Austria that provides world-wide personalised solutions for electron microscopy, including training, consulting, and software.
Immersion Freezing for Cryo-Transmission Electron Microscopy: Applications
A well established usage case for cryo-TEM is three-dimensional reconstruction of isolated macromolecules, virus particles, or filaments. On one hand, these approaches are based on averaging of…
Immersion Freezing for Cryo-Transmission Electron Microscopy: Fundamentals
The high vacuum required in a transmission electron microscope (TEM) greatly impairs the ability to study specimens naturally occurring in an aqueous phase: exposing "wet" specimens to a pressure…
Glycerol Spraying/Platinum Low Angle Rotary Shadowing of DNA with the Leica EM ACE600 e-beam
Glycerol spraying/low angle rotary shadowing (Aebi and Baschong, 2006) is a preparation technique used in biology to visualize structures yielding insufficent contrast with other techniques, due to…
Perusing Alternatives for Automated Staining of TEM Thin Sections
Contrast in transmission electron microscopy (TEM) is mainly produced by electron scattering at the specimen: Structures that strongly scatter electrons are referred to as electron dense and appear as…