Autiva Spatial Phenotyper

Autiva Spatial Phenotyper is an end-to-end spatial biology workflow that integrates staining chemistry, image acquisition, and data analysis into one consistent platform for scientific work across academic and pharma research laboratories. It combines automated imaging with microfluidic-driven reagent cycling to help users move from sample preparation through iterative multiplex imaging and analysis with minimal manual intervention.

Autiva Spatial Phenotyper is intended for use in both academic and pharmaceutical research settings. It is designated for Research Use Only (RUO).

The system supports walk-away execution of high-plex experiments, including automated cyclic staining and imaging workflows. After setup, users can run automated multi-cycle staining and imaging over multiple days, reducing the need for repeated manual interaction.

Yes. The system is designed to execute cyclic staining and de-staining workflows and supports automated cyclic staining and de-staining operations, enabling reproducible multi-cycle experimental workflows with reduced manual interaction.

The system automatically detects samples and identifies regions of interest, minimizing manual setup and improving workflow efficiency.

Autiva Spatial Phenotyper supports experiments with one to four slides, enabling users to scale from pilot studies to larger experiments without changing platform configuration.

The system enables whole-slide imaging over an area of 25 × 17 mm per slide, supporting comprehensive analysis of large tissue sections.

For high-throughput workflows, the system can complete staining, imaging, and processing of four tissue samples measuring 15 × 15 mm with 60 biomarkers each within 48 hours. For single-slide workflows, the system can image and process a full tissue slide measuring 15 × 15 mm with 20-plex within 8 hours, excluding staining.

The system supports antibody volumes of 220–250 µL and is designed to minimize reagent waste with dead volumes below 1% per well, supporting efficient use of high cost consumables.

Yes. The system allows reagent replenishment during operation, which supports uninterrupted execution of large-scale experiments such as workflows above 60 biomarkers.

The system maintains reagents within specified temperature ranges from 4°C to 40°C and protects them from contamination, evaporation, and light exposure, helping preserve reagent integrity throughout experiments.

The system preserves sample integrity with less than 1% degradation after high-plex experiments, supporting reliable downstream analyses.

The system supports THUNDER imaging functionality in 2D to help enhance signal-to-noise ratio and improve image quality for advanced biological analysis. It also includes calibration and quality control mechanisms for robust imaging performance.

Yes. The systems enables imaging at cellular resolution for accurate cell-level profiling and subcellular resolution for visualizing structures such as nuclei, membranes with high spatial precision.

Users can select pre-defined biomarker panels for use during protocol creation. Once panels are selected, panel information can be pre-populated into the protocol publisher tools, including biomarkers assigned to rounds and channels, exposure times, and the channel used for nuclear identification.

The fluidics system is designed to enable a variety of fluidics-based research workflows beyond ATTOAuriga. While the underlying pump performance is validated, users are responsible for developing and validating any custom protocols.