Viventis Dual View Light Sheet Fluorescence Microscope

Revealing life in its full context

Detection and Illumination optics	Illumination: two 10X objectives, NA 0.2. Detection: two 25X NA 1.1 OR two 16X NA 0.8 water immersion objectives.
Illumination	External laser combiner with a maximum of six wavelengths (from 405nm to 685nm), Omicron LightHUB+. Transmitted light illumination (LED light source) to locate the sample and acquire transmitted images.
Light sheet specification	Light sheet generated by scanning of a Gaussian beam. Three switchable light-sheets with thicknesses (FWHM) of approximately 2.3, 4 and 7 μm for different sample sizes. Automatic position-specific light-sheet alignment.
Detection camera	Two high sensitivity Hamamatsu ORCA-Fusion USB3 cameras. Field of view: 16X Objective: 536µm; 25X Objective: 599µm Pixel size: 16X Objective: 406nm; 25X Objective: 260nm
Sample part	Motorized XYZ sample stage with maximum X travel range of 50mm Multiple sample located in an open-top FEP sample chamber (multi-well).
Sample incubation	Recirculating air temperature controller from 5°C above ambient temperature to 40°C. CO2 concentration control range 2-10%. O2 concentration control range 5-20%.

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