Outstanding reproducibility and sample quality

Automatic Plunge Freezer EM GP2

The updated version of the EM GP automatic plunge freezer is based on our motto ‘with the users for the user’ in combining performance and flexibility in a practically-relevant way.

The EM GP2 plunge freezes fluid or extremely thin samples spread on an electron microscopy grid into liquid ethane after removing excess fluid by automatic blotting.

Prior to freezing the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between +4°C and +60°C and room humidity to 99 %. 

EM GP2
Automatic Plunge Freezer EM GP2
Microtubules acquired at 20000x
Microtubules acquired at 39000x

Reproducible Results Within Minutes

Micrographs A and B show images of microtubules acquired at 20000x (A) and 39000x (B) nominal magnification in the hiles of the perforated carbon film. The ice is free of exogenous contamination, the protofilaments and the individual tubulin subunits are clearly visible along the length of the microtubules.

Courtesy of Dr. Guenter Resch, Nexperion e.U. - Solutions for Electron Microscopy; Dr. Thomas Heuser and Marlene Brandstetter, Vienna, Biocenter Core Facilities GmbH

User Safety 

Safe and reproducible preparation for single particle analysis and cryo-tomography.

  • Special rapid condensing liquefier head for easy and save fill of secondary cryogen
  • Fixed secondary cryogen position, only environmental chamber movement

Reproducibility

Freezing grids reproducibly and safely using the EM GP2 automatic plunge freezer.

  • Sensor blotting gives equal distributes surface on grid for perfect imaging results
  • Temperature stability of secondary cryogen
  • Temperature and humidity controlled protected environment

Connectivity

To be connected at any time at any place is today something which is taken for granted.

As a logical consequence Leica Microsystems started to connect systems for EM Sample Preparation to ensure your samples are protected during the complete workflow process.

Sample Safety

  • Dry Nitrogen Gas Flow environment
  • Sample Storage and Transfer in liquid face using transfer container with pre-cooled grid box
  • Customized adapter for optimized and safe sample transfer from the automatic plunge freezer EM GP2 to different cryo TEMs

Flexibility

Depending on your needs the EM GP2 automatic plunge freezer can be configured individually by using different blotting types and parameters:

  • Sensor controlled blotting
  • Automated or manual single, double and multiple blotting
  • Environmental parameter – Temperature and Humidity
  • Blotting Parameter – Pre-Blot, Blot, Post-Blot time, Blot and Transfer Positions

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?

  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Service Phone : 1 800 248 0223
    Fax : +1 847-236-3009
    Routeplanner (google maps)
  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Service Phone : 1 800 248 0223
    Fax : +1 847-236-3009
    Routeplanner (google maps)

You will find a more detailed list of local contacts here.

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