Andreia Pinto , Dr.

Advanced Workflow Specialist, Leica Microsystems

Dr. Pinto started her career as a Biomedical Scientist in Histocellular Pathology with a particular interest in electron microscopy. In 2014, she worked at the Primary Ciliary Diagnosis (PCD) in Lisbon. In 2019, Andreia moved to the Royal Brompton Hospital, London, working as a Thoracic Research Associate, where she was responsible for training a deep machine learning platform to recognize patterns in EM images of cilia in diagnosing PCD. She completed her PhD on this topic in 2022 while also investigating new insights into SARS-CoV-2 infection of the respiratory airway. Andreia is currently an Advanced Workflow Application Specialist with Leica Microsystems, based at the Imaging Centre of the European Molecular Biology Laboratory (EMBL) in Heidelberg.

Waffle method lamella preparation and cryo-ET results - Representative workflow and results of lamella preparation from a vitrified C. elegans L1 larva using the waffle method. Image courtesy: Prof. Dr. Jürgen M. Plitzko - doi.org/10.1038/s41592-023-02113-5-

Waffle Method Workflow: From HPF to Cryo-ET Lamellae

Waffle freezing provides an HPF-based route to cryo-ET sample preparation. This workflow guide follows the process from grid and carrier assembly to vitrification, cryo-FIB milling, lamella…

Complete camera overview of EM grid recorded with 3 channels. Inserts displaying the positions, where superresolved 3D confocal images were recorded. 3D renderings of these positions are shown in the zoomed inserts. Fluorescence channels (nuclei by Hoechst, blue; mitochondria by MitoTracker Green, green; lipid Droplets by Bodipy and Crimson Beads, red). Width of a grid square is 90 ?m, width of a grid bar is 35 ?m. Samples kindly provided by Ievgeniia Zagoriy, Mahamid-Group, EMBL Heidelberg, Germany.

From Bench to Beam: A Complete Correlative Cryo Light Microscopy Workflow

In the webinar entitled "A Multimodal Vitreous Crusade, a Cryo Correlative Workflow from Bench to Beam" a team of experts discusses the exciting world of correlative workflows for structural biology…