The contents of this application note are the following:
- Why microscope calibration is important
Accuracy and reliability of an instrument’s measurements, how calibration verifies them, what influences accurate and reliable results, and fulfilling guidelines and standards are all discussed.
- How to calibrate a microscope
Different types of optical microscopes are available, such as stereo, compound, and digital, but today very often measurements are made using a digital microscope camera and software, rather than an eyepiece reticle.
- Step-by-step calibration guide
Calibration of a microscope with a digital camera installed using a stage micrometer as the measurement reference is described in detail. A total of 6 steps, including the generation of a calibration report, is shown.
- Useful microscope accessories for calibration
Accessories can help when performing microscope calibration, such as, for cleaning the optics and making precise horizontal stage movements.
- Professional calibration service
A service for periodic calibration of the microscope is available from Leica Microsystems.
- Summary and Conclusions
By comparing and adjusting microscope measurements to a known reference or standard, calibration helps guarantee accurate and reliable results over time and for multiple users, increasing confidence in the measurements. Calibration can also be required for accordance with international, regional, or organizational guidelines or standards. A calibrated microscope can be essential for certain applications and industries, like inspection, QC, failure analysis, R&D, scientific research and the automotive, aerospace, electronics, and medical device industries. Microscope calibration can be done either by users directly or they can take advantage of professional services offered by Leica Microsystems. It is recommended that calibration be done right after microscope installation, repair, or upgrade, and repeated at least once a year.
