Sciences de la vie
Sciences de la vie
C'est ici que vous pourrez développer vos connaissances, vos capacités de recherche et les applications pratiques de la microscopie dans divers domaines scientifiques. Apprenez à obtenir une visualisation précise, à interpréter les images et à faire progresser la recherche. Trouvez des informations pertinentes sur la microscopie avancée, les techniques d'imagerie, la préparation des échantillons et l'analyse des images. Les sujets abordés comprennent la biologie cellulaire, les neurosciences et la recherche sur le cancer, en mettant l'accent sur les applications et les innovations de pointe.
What is FRET with FLIM or as it is usually known FLIM-FRET?
Förster resonance energy transfer (FRET) is a well-established fluorescence-based technique which is used to study molecular interactions. It is useful for the analysis of protein-DNA and…
Ratiometric Imaging and Analysis of Ion Concentration in Cells
Many cellular functions depend on the dynamic balance of ions, electric potentials, and pH between the cytosol and surrounding extracellular space. Changes in these values affect cellular function.…
High-Pressure Freezing Protocols for Ultrastructural 3D EM
High pressure freezing (HPF) can help preserve hydrated cells and tissues close to their biological state at the moment of immobilization, supporting more reliable ultrastructural interpretation than…
Ultramicrotome UC Enuity in Practice: Stable 15 nm Sections at ZFE
After using the UCT and UC6 ultramicrotomes, Claudia Mayrhofer calls UC Enuity a leap in stability—so robust that vibrations and temperature shifts don’t spoil sections, even with multiple users. Auto…
Eliminating Electrostatic Interference in Laser Microdissection
Electrostatic charge in laser microdissection (LMD) causes two critical failures: samples stick to charged surfaces and are lost, or samples fly into adjacent wells and cause cross-contamination. We…
History, Developments and Trends of Microscopy in Cancer Research
Cancer is a global disease, with 18 million new cases diagnosed and 10 million cancer-related deaths worldwide in 2020. This burden is set to increase, with a projected increase in cases of ~55% by…
Overview of Fluorescent Dyes in terms of Applications and Properties
An introduction to commonly used fluorescent dyes and an overview of their characteristics are given in this article. Fluorescence microscopy is used for the study of specific cellular components with…
Researchers Insights: Microscopy in Cancer Research
Discover how imaging techniques are driving cancer research forward. In this issue, we present comprehensive multimodal studies using microscopy, as well as new directions in intraoperative cancer…
A Guide to Fluorescence Microscopy
Fluorescence microscopy uses the ability of fluorophores, dyes, or fluorescent proteins to emit light of a specific wavelength after being excited with light of a shorter wavelength. Biomolecules can…
