ATTO 514

ATTO 514 is a new hydrophilic fluorescent label with excellent water solubility.

Optical properties

λ _abs = 511 nm
ε _max = 1.15×10⁵ M ^-1 cm ^-1
λ _fl = 532 nm
n _fl = 85 %
τ _fl = 3.9 ns
CF ₂₆₀ = ε ₂₆₀ /ε _max = 0.21
CF ₂₈₀ = ε ₂₈₀ / ε _max = 0.07

ATTO 514 is a new hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield, and exceptional thermal and photo-stability. Thus ATTO 514 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.

The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is the 514 nm line of an Argon-Ion laser.

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

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