Leica DMI6000 B with Adaptive Focus Control
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AFC switched on
COS cells were transfected with a Golgi specific GFP variant. The complete microscope system was placed in a climate chamber with a temperature of 37°C. Shortly after starting the acquisition 1ml of…
AFC switched off
COS cells were transfected with a Golgi specific GFP variant. The complete microscope system was placed in a climate chamber with a temperature of 37°C. Shortly after starting the acquisition 1ml of…
TIRF imaging with simultaneous AFC
MDCK cells expressing GFP-p75Courtesy of Prof. Dr. Ralf Jacob and Dr. Alexandra Elli, Institute of Cytobiology and Cytopathology, University of Marburg, Germany.