Leica LMD6 & LMD7 Laser Microdissection Microscopes
Dissection Perfection
This list presents current publications in the major application fields for laser microdissection and is updated monthly.
Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.
The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.
Download the complete list of LMD publications in "Downloads"
Single Cell Applications
Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E
Cancer Cell, 26 June 2025
Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics
Zheng X, Mund A, Mann M
STAR Protoc. 2025 Jun 18;6(3):103901. doi: 10.1016/j.xpro.2025.103901.
Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025
DNA Research
Age-dependent accumulation of mitochondrial tRNA mutations in mouse kidneys linked to mitochondrial kidney diseases
Zhang L, Xu Z, Jing J, Chai G, Xie G, Ru Y, Lv Q, Zuo X, Zhang Q, Chen J, Jin H, Liu N, Kong M, Shen B, Liu M, Jiang L, Wang X, Zhang Y, Jiang M
Nat Aging. 2025 Jun 27. doi: 10.1038/s43587-025-00909-y.
Attenuated immune surveillance during squamous cell transformation of pancreatic adenosquamous cancer defines new therapeutic opportunity for cancer interception
Chen X, Sun S, Li S, Yu S, Chen J, Chen X
J Immunother Cancer. 2025 Jun 23;13(6):e012066. doi: 10.1136/jitc-2025-012066.
Comparison of Somatic Mutations in Primary Squamous Cell Carcinomas of the Hypopharynx and the Esophagus Developed in Individual Patients
Matsui H, Eguchi H, Okazaki Y, Hirayama Y, Nibu KI, Shinomiya H
Head Neck. 2025 Jun 19. doi: 10.1002/hed.28220.
RNA Research
Attenuated immune surveillance during squamous cell transformation of pancreatic adenosquamous cancer defines new therapeutic opportunity for cancer interception
Chen X, Sun S, Li S, Yu S, Chen J, Chen X
J Immunother Cancer. 2025 Jun 23;13(6):e012066. doi: 10.1136/jitc-2025-012066.
Sex- and subregion-specific vulnerability of the hippocampus and amygdala to intermittent hypoxia in relation to learning/memory function and anxiety tendencies of infant rats
Takenouchi Y, Hosomichi J, Angkanawaraphan K, Maeda H, Hong H, Changsiripun C, Ono T
Sleep Breath. 2025 Jun 9;29(3):209. doi: 10.1007/s11325-025-03382-4.
ciRS-7 expression is epigenetically regulated in cancer cells across human adenocarcinomas
Paasch TP, Jarlstad Olesen MT, García-Rodríguez JL, Assmus AM, Fenton RA, Kjems J, Hager H, Kristensen LS
PLoS Genet. 2025 Jun 2;21(6):e1011726. doi: 10.1371/journal.pgen.1011726.
Proteomics, Lipidomics, Metabolomics
Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E
Cancer Cell, 26 June 2025
Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics
Zheng X, Mund A, Mann M
STAR Protoc. 2025 Jun 18;6(3):103901. doi: 10.1016/j.xpro.2025.103901.
An iPSC-derived CD19/BCMA CAR-NK therapy in a patient with systemic sclerosis
Wang X, Zhang Y, Jin Y, Dai L, Yue Y, Hu J, Liu X, Pang K, Ye S, Chen Y, Ye W, Shi X, Ma X, Guo L, Liu Y, Ta N, Zhu X, Lin L, Wang J, Yan R, Wang P, Song X, Zhou Y, Zhou L, Wang Q, Guan T, Li T, Zhou L, Pan W, He Y, Wu X, Xu Y, Yang L, Xu H
Cell. 2025 Jun 16:S0092-8674(25)00625-7. doi: 10.1016/j.cell.2025.05.038.
The melanoma MEGA-study: Integrating proteogenomics, digital pathology, and AI-analytics for precision oncology
Guedes J, Szadai L, Woldmar N, Jánosi ÁJ, Koroncziová K, Lengyel BM, Kelemen B, Boltas E, Gyulai R, Wieslander E, Pawlowski K, Horvatovich P, Betancourt L, Szasz AM, Vereb Z, Horvath P, Oskolás H, Appelqvist R, Malm J, Marko-Varga G, Németh IB, Gil J
J Proteomics. 2025 Jun 16:105482. doi: 10.1016/j.jprot.2025.105482.
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Highlight 2025:
Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4
Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2
Highlights 2024:
Method of the Year 2024: spatial proteomics.
Nat Methods 21, 2195–2196 (2024).
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409
Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Fluorescence Applications
Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E
Cancer Cell, 26 June 2025
Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025
Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.
Cancer Research
Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E
Cancer Cell, 26 June 2025
Attenuated immune surveillance during squamous cell transformation of pancreatic adenosquamous cancer defines new therapeutic opportunity for cancer interception
Chen X, Sun S, Li S, Yu S, Chen J, Chen X
J Immunother Cancer. 2025 Jun 23;13(6):e012066. doi: 10.1136/jitc-2025-012066.
Comparison of Somatic Mutations in Primary Squamous Cell Carcinomas of the Hypopharynx and the Esophagus Developed in Individual Patients
Matsui H, Eguchi H, Okazaki Y, Hirayama Y, Nibu KI, Shinomiya H
Head Neck. 2025 Jun 19. doi: 10.1002/hed.28220.
The melanoma MEGA-study: Integrating proteogenomics, digital pathology, and AI-analytics for precision oncology
Guedes J, Szadai L, Woldmar N, Jánosi ÁJ, Koroncziová K, Lengyel BM, Kelemen B, Boltas E, Gyulai R, Wieslander E, Pawlowski K, Horvatovich P, Betancourt L, Szasz AM, Vereb Z, Horvath P, Oskolás H, Appelqvist R, Malm J, Marko-Varga G, Németh IB, Gil J
J Proteomics. 2025 Jun 16:105482. doi: 10.1016/j.jprot.2025.105482.
Neurosciences
Sex- and subregion-specific vulnerability of the hippocampus and amygdala to intermittent hypoxia in relation to learning/memory function and anxiety tendencies of infant rats
Takenouchi Y, Hosomichi J, Angkanawaraphan K, Maeda H, Hong H, Changsiripun C, Ono T
Sleep Breath. 2025 Jun 9;29(3):209. doi: 10.1007/s11325-025-03382-4.
Emergence of the sex difference in calbindin D-28K neurons and the role of calbindin D-28K in clustering neurons of the preoptic area in mice
Koiso R, Kushida C, Kanaya M, Tsukahara S
Cell Tissue Res. 2025 May 21. doi: 10.1007/s00441-025-03981-3.
Single-cell genotyping and transcriptomic profiling of mosaic focal cortical dysplasia
Baldassari S, Klingler E, Teijeiro LG, Doladilhe M, Raoux C, Roig-Puiggros S, Bizzotto S, Couturier J, Gilbert A, Sami L, Ribierre T, Aronica E, Adle-Biassette H, Chipaux M, Jabaudon D, Baulac S
Nat Neurosci. 2025 Apr 30. doi: 10.1038/s41593-025-01936-z.
Clinical/Pathology/Immunology Research Applications
Lesion penetration predicts tuberculosis regimen success: a validated translational tool for de-risking late-stage drug development
Savic R, Ernest J, Bustion A, Patel A, Sarathy J, Kaya F, Rodriguez S, Strydom N, Silva C, Flori L, Vu H-A, Westergren V, Via L, Dartois V
Research Square, 25th June 2025
Laser Microdissection and Mass Spectrometry Reveals Diverse Membranous Nephropathy Antigens
Inácio A, Domingues P, Piedade A, Mendes B, Fonseca F, Vrana J, Theis J, Fervenza FC, Soto K, Sethi S
Kidney International Reports, 23 June 2025
An iPSC-derived CD19/BCMA CAR-NK therapy in a patient with systemic sclerosis
Wang X, Zhang Y, Jin Y, Dai L, Yue Y, Hu J, Liu X, Pang K, Ye S, Chen Y, Ye W, Shi X, Ma X, Guo L, Liu Y, Ta N, Zhu X, Lin L, Wang J, Yan R, Wang P, Song X, Zhou Y, Zhou L, Wang Q, Guan T, Li T, Zhou L, Pan W, He Y, Wu X, Xu Y, Yang L, Xu H
Cell. 2025 Jun 16:S0092-8674(25)00625-7. doi: 10.1016/j.cell.2025.05.038.
Modulation of podocyte extracellular matrix remodeling in membranous nephropathy by the NFATc3/LRRC55/BK channel pathway
Guo Y, Min J, Chang B, Liu L, Zhang J, Chen W
J Cell Commun Signal. 2025 Jun 13;19(2):e70022. doi: 10.1002/ccs3.70022.
Developmental Research, Epigenetics & Aging
Age-dependent accumulation of mitochondrial tRNA mutations in mouse kidneys linked to mitochondrial kidney diseases
Zhang L, Xu Z, Jing J, Chai G, Xie G, Ru Y, Lv Q, Zuo X, Zhang Q, Chen J, Jin H, Liu N, Kong M, Shen B, Liu M, Jiang L, Wang X, Zhang Y, Jiang M
Nat Aging. 2025 Jun 27. doi: 10.1038/s43587-025-00909-y.
ciRS-7 expression is epigenetically regulated in cancer cells across human adenocarcinomas
Paasch TP, Jarlstad Olesen MT, García-Rodríguez JL, Assmus AM, Fenton RA, Kjems J, Hager H, Kristensen LS
PLoS Genet. 2025 Jun 2;21(6):e1011726. doi: 10.1371/journal.pgen.1011726.
Profiling (placental) DNA methylation in cell-free DNA across gestation: the Rotterdam Periconception Cohort
van Vliet MM, Boers RG, Boers JB, Schäffers OJM, van der Meeren LE, Gribnau J, Schoenmakers S, Steegers-Theunissen RPM
Mol Hum Reprod. 2025 May 8:gaaf011. doi: 10.1093/molehr/gaaf011
Plant Research
RsLBD3 regulates the secondary growth of taproot by integrating auxin and cytokinin signaling in radish (Raphanus sativus L.)
Dong J, Wang Y, Xu L, Li B, Zhang X, Chen Y, Ying J, Chen S, Cui F, Liu L
J Integr Plant Biol. 2025 May 7. doi: 10.1111/jipb.13918.
Laser microdissection and fluorescence in situ hybridization reveal the tissue-specific gene expression in the ovules of P. tabulaeformis Carr
Zhang X, Chen B, Song X, Wang Y, Zheng C, Gong Z
J Plant Physiol. 2025 Apr 22:309:154500. doi: 10.1016/j.jplph.2025.154500.
Role of an endodermis-specificmiR858b-MYB1Lmodule inthe regulation of Taxol biosynthesis inTaxus mairei
Yu C, Zhang D, Zhang L, Fang Z, Zhang Y, Lin W, Ma R, Zheng M, Bai E, Shen
The plant journal, Volume122, Issue1, 05 April 2025, e70135
Forensics
Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.
Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807
Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136
General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/
Miscellaneous
Methane-powered sea spiders: Diverse, epibiotic methanotrophs serve as a source of nutrition for deep-sea methane seep Sericosura
Dal Bó B, Guo Y, Mayr MJ, Pereira OS, Levin LA, Orphan VJ, Goffredi SK
Proc Natl Acad Sci U S A. 2025 Jul;122(26):e2501422122. doi: 10.1073/pnas.2501422122.
Micromorphological features of the structure of the tooth pulp chamber wall in dogs
Frolov VV, Egunova AV, Ivantsov VA
International Journal of Veterinary Medicine. 2025;(1):222-227. (In Russ.)
Inhibiting 5-HT2C Receptor in the Hippocampus Rescues EphB2-Dependent Memory Impairment in Mice
Jana S, Chandran SK, Rosenblum K, Lamprecht R
European Journal of Pharmacology, doi.org/10.1016/j.ejphar.2025.177729.
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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23
Live Cell Culture
A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX
A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023
SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416
