Leica LMD6 & LMD7 Laser Microdissection Microscopes

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression 
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E
Cancer Cell, 26 June 2025

Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomics
Zheng X, Mund A, Mann M
STAR Protoc. 2025 Jun 18;6(3):103901. doi: 10.1016/j.xpro.2025.103901.

An ultrasensitive spatial tissue proteomics workflow exceeding 100 proteomes per day
Klingeberg M, Krisp C, Fritzsche S, Schallenberg S, Hornburg D, and Coscia F
bioRxiv, 05 June 2025

Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025

DNA Research

Mutation profile and chromosomal abnormality in adenomyosis
Suda K, Takahashi K, Tamura R, Saito K, Yamaguchi M, Yachida N, Adachi S, Kase H, Okuda S, Yoshihara K, and Nakaoka H
Reproduction, 07 Jul 2025, REP-25-0132

Two-hit events occurred independently in bilateral breast cancers in a germline double heterozygous carrier for BRCA1 and BRCA2
Semba R, Eguchi H, Takatsu M, Hashizume T, Moteki H, Maeno K, Murakami F, Watanabe J, Kutomi G, Arai M
Breast Cancer. 2025 Jul 2. doi: 10.1007/s12282-025-01740-4.

Age-dependent accumulation of mitochondrial tRNA mutations in mouse kidneys linked to mitochondrial kidney diseases
Zhang L, Xu Z, Jing J, Chai G, Xie G, Ru Y, Lv Q, Zuo X, Zhang Q, Chen J, Jin H, Liu N, Kong M, Shen B, Liu M, Jiang L, Wang X, Zhang Y, Jiang M
Nat Aging. 2025 Jun 27. doi: 10.1038/s43587-025-00909-y.

RNA Research

Gene expression landscape of the Brassica napus seed reveals subgenome bias in both space and time.
Ziegler DJ, Khan D, Kalichuk JL, Becker MG, Pasha A, Provart NJ, Belmonte MF
Plant Physiol. 2025 Jun 28:kiaf283. doi: 10.1093/plphys/kiaf283.

Attenuated immune surveillance during squamous cell transformation of pancreatic adenosquamous cancer defines new therapeutic opportunity for cancer interception
Chen X, Sun S, Li S, Yu S, Chen J, Chen X
J Immunother Cancer. 2025 Jun 23;13(6):e012066. doi: 10.1136/jitc-2025-012066.

Sex- and subregion-specific vulnerability of the hippocampus and amygdala to intermittent hypoxia in relation to learning/memory function and anxiety tendencies of infant rats
Takenouchi Y, Hosomichi J, Angkanawaraphan K, Maeda H, Hong H, Changsiripun C, Ono T
Sleep Breath. 2025 Jun 9;29(3):209. doi: 10.1007/s11325-025-03382-4.

Proteomics, Lipidomics, Metabolomics

AI-powered Deep Visual Proteomics reveals critical molecular transitions in pancreatic cancer precursors
Min J, Schweizer L, Zonderland G, Selvanesan BC, Oldenburg L, Bae S-W, Kim BJ, Swanson BJ, Klute KA, Caffrey TC, Grandgenett PM, Hollingsworth MA, Ummat I, Strauss MT, Mund A, Maitra A
bioRxiv, 7 Jul 2025

Pancreatic Neuroendocrine Tumors Secrete Apolipoprotein E to Induce Tip Endothelial Cells That Remodel the Tumor-Stroma Ratio and Promote Cancer Progression
Lou X, Shi Y, Zhao F, Xu X, Wang Y, Qin Y, Zhang W, Ye Z, Wang F, Ding T, Jing D, Fan G, Zhang Y, Chen X, Chen J, Yu X, Xu J, Ji S
Cancer Res. 2025 Jul 7:OF1-OF15. doi: 10.1158/0008-5472.CAN-24-2528.

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E 
Cancer Cell, 26 June 2025

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Highlight 2025: 

Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression 
Schweizer L, Kenny HA, Krishnan R, Kelliher L, Bilecz AJ, Heide J, Donle L, Shimizu A, Metousis A, Mendoza R, Nordmann TM, Rauch S, Richter S, Yan L, Rosenberger FA, Strauss MT, Kurnit KC, Thielert M, Rodriguez E, Müller-Reif JB, Yamada SD, Theis FJ, Mund A, Lastra RR, Mann M, and Lengyei E
Cancer Cell, 26 June 2025

Multiplexed Deep Visual Proteomics Unveils Spatial Heterogeneity and Rare Endocrine States in Human Adult Pancreatic Islets
Mardamshina M, Dorka N, Thielert M, Bjoerklund F, Navarro FB, Casals AM, Kovacs F, Fox JEM, Horvath P, Macdonald PE, Mann M, Lundberg
bioRxiv 27 April 2025

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

Cancer Research

Pancreatic Neuroendocrine Tumors Secrete Apolipoprotein E to Induce Tip Endothelial Cells That Remodel the Tumor-Stroma Ratio and Promote Cancer Progression
Lou X, Shi Y, Zhao F, Xu X, Wang Y, Qin Y, Zhang W, Ye Z, Wang F, Ding T, Jing D, Fan G, Zhang Y, Chen X, Chen J, Yu X, Xu J, Ji S
Cancer Res. 2025 Jul 7:OF1-OF15. doi: 10.1158/0008-5472.CAN-24-2528.

AI-powered Deep Visual Proteomics reveals critical molecular transitions in pancreatic cancer precursors
Min J, Schweizer L, Zonderland G, Selvanesan BC, Oldenburg L, Bae S-W, Kim BJ, Swanson BJ, Klute KA, Caffrey TC, Grandgenett PM, Hollingsworth MA, Ummat I, Strauss MT, Mund A, Maitra A
bioRxiv, 7 Jul 2025

Mutation profile and chromosomal abnormality in adenomyosis
Suda K, Takahashi K, Tamura R, Saito K, Yamaguchi M, Yachida N, Adachi S, Kase H, Okuda S, Yoshihara K, and Nakaoka H
Reproduction, 07 Jul 2025, REP-25-0132

Two-hit events occurred independently in bilateral breast cancers in a germline double heterozygous carrier for BRCA1 and BRCA2
Semba R, Eguchi H, Takatsu M, Hashizume T, Moteki H, Maeno K, Murakami F, Watanabe J, Kutomi G, Arai M
Breast Cancer. 2025 Jul 2. doi: 10.1007/s12282-025-01740-4.

Neurosciences

Sex- and subregion-specific vulnerability of the hippocampus and amygdala to intermittent hypoxia in relation to learning/memory function and anxiety tendencies of infant rats
Takenouchi Y, Hosomichi J, Angkanawaraphan K, Maeda H, Hong H, Changsiripun C, Ono T
Sleep Breath. 2025 Jun 9;29(3):209. doi: 10.1007/s11325-025-03382-4.

Emergence of the sex difference in calbindin D-28K neurons and the role of calbindin D-28K in clustering neurons of the preoptic area in mice
Koiso R, Kushida C, Kanaya M, Tsukahara S
Cell Tissue Res. 2025 May 21. doi: 10.1007/s00441-025-03981-3.

Single-cell genotyping and transcriptomic profiling of mosaic focal cortical dysplasia
Baldassari S, Klingler E, Teijeiro LG, Doladilhe M, Raoux C, Roig-Puiggros S, Bizzotto S, Couturier J, Gilbert A, Sami L, Ribierre T, Aronica E, Adle-Biassette H, Chipaux M, Jabaudon D, Baulac S
Nat Neurosci. 2025 Apr 30. doi: 10.1038/s41593-025-01936-z.

Clinical/Pathology/Immunology Research Applications

Lesion penetration predicts tuberculosis regimen success: a validated translational tool for de-risking late-stage drug development
Savic R, Ernest J, Bustion A, Patel A, Sarathy J, Kaya F, Rodriguez S, Strydom N, Silva C, Flori L, Vu H-A, Westergren V, Via L, Dartois V
Research Square, 25th June 2025

Laser Microdissection and Mass Spectrometry Reveals Diverse Membranous Nephropathy Antigens
Inácio A, Domingues P, Piedade A, Mendes B, Fonseca F, Vrana J, Theis J, Fervenza FC, Soto K, Sethi S
Kidney International Reports, 23 June 2025

An iPSC-derived CD19/BCMA CAR-NK therapy in a patient with systemic sclerosis
Wang X, Zhang Y, Jin Y, Dai L, Yue Y, Hu J, Liu X, Pang K, Ye S, Chen Y, Ye W, Shi X, Ma X, Guo L, Liu Y, Ta N, Zhu X, Lin L, Wang J, Yan R, Wang P, Song X, Zhou Y, Zhou L, Wang Q, Guan T, Li T, Zhou L, Pan W, He Y, Wu X, Xu Y, Yang L, Xu H
Cell. 2025 Jun 16:S0092-8674(25)00625-7. doi: 10.1016/j.cell.2025.05.038.

Modulation of podocyte extracellular matrix remodeling in membranous nephropathy by the NFATc3/LRRC55/BK channel pathway
Guo Y, Min J, Chang B, Liu L, Zhang J, Chen W
J Cell Commun Signal. 2025 Jun 13;19(2):e70022. doi: 10.1002/ccs3.70022.

Developmental Research, Epigenetics & Aging

Age-dependent accumulation of mitochondrial tRNA mutations in mouse kidneys linked to mitochondrial kidney diseases
Zhang L, Xu Z, Jing J, Chai G, Xie G, Ru Y, Lv Q, Zuo X, Zhang Q, Chen J, Jin H, Liu N, Kong M, Shen B, Liu M, Jiang L, Wang X, Zhang Y, Jiang M
Nat Aging. 2025 Jun 27. doi: 10.1038/s43587-025-00909-y.

ciRS-7 expression is epigenetically regulated in cancer cells across human adenocarcinomas
Paasch TP, Jarlstad Olesen MT, García-Rodríguez JL, Assmus AM, Fenton RA, Kjems J, Hager H, Kristensen LS
PLoS Genet. 2025 Jun 2;21(6):e1011726. doi: 10.1371/journal.pgen.1011726.

Profiling (placental) DNA methylation in cell-free DNA across gestation: the Rotterdam Periconception Cohort
van Vliet MM, Boers RG, Boers JB, Schäffers OJM, van der Meeren LE, Gribnau J, Schoenmakers S, Steegers-Theunissen RPM
Mol Hum Reprod. 2025 May 8:gaaf011. doi: 10.1093/molehr/gaaf011

Plant Research

Gene expression landscape of the Brassica napus seed reveals subgenome bias in both space and time.
Ziegler DJ, Khan D, Kalichuk JL, Becker MG, Pasha A, Provart NJ, Belmonte MF
Plant Physiol. 2025 Jun 28:kiaf283. doi: 10.1093/plphys/kiaf283.

RsLBD3 regulates the secondary growth of taproot by integrating auxin and cytokinin signaling in radish (Raphanus sativus L.)
Dong J, Wang Y, Xu L, Li B, Zhang X, Chen Y, Ying J, Chen S, Cui F, Liu L
J Integr Plant Biol. 2025 May 7. doi: 10.1111/jipb.13918.

Laser microdissection and fluorescence in situ hybridization reveal the tissue-specific gene expression in the ovules of P. tabulaeformis Carr
Zhang X, Chen B, Song X, Wang Y, Zheng C, Gong Z
J Plant Physiol. 2025 Apr 22:309:154500. doi: 10.1016/j.jplph.2025.154500.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Methane-powered sea spiders: Diverse, epibiotic methanotrophs serve as a source of nutrition for deep-sea methane seep Sericosura
Dal Bó B, Guo Y, Mayr MJ, Pereira OS, Levin LA, Orphan VJ, Goffredi SK
Proc Natl Acad Sci U S A. 2025 Jul;122(26):e2501422122. doi: 10.1073/pnas.2501422122.

An ultrasensitive spatial tissue proteomics workflow exceeding 100 proteomes per day
Klingeberg M, Krisp C, Fritzsche S, Schallenberg S, Hornburg D, and Coscia F
bioRxiv, 05 June 2025

Micromorphological features of the structure of the tooth pulp chamber wall in dogs
Frolov VV, Egunova AV, Ivantsov VA
International Journal of Veterinary Medicine. 2025;(1):222-227. (In Russ.)

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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416