LAS X Life Science
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Leica Microsystems
LAS X Life Science Microscope Software Platform
One for all
Supported Hardware (Widefield systems) | Short description |
| Leica microscope cameras | DFC7000 T, DFC7000 GT, K8, K5, K5C, K3C, K3M, DFC9000 GT, DFC9000 GTC, DFC3000 G, DMC2900, DMC4500, DMC5400, C1, C3, DMC6200, DFC450 C, DFC295, IC90E, ICC50W, ICC50E |
| Andor microscope cameras | iXonUltra 888, iXonUltra Life 897, iXonUltra 897 |
| Hamamatsu microscope cameras | Flash 4.0 V2 CL, Flash 4.0 V2 USB, Flash 4.0 V3 CL, Flash 4.0 V3 USB |
| Photometrics microscope cameras | Prime 95B PCIe, Prime 95B USB3 |
| Fluorescence light sources | LED8, LED5, LED3, LED1, EL6000, Lumen Dynamics X-Cite 200 DC, Lumen Dynamics X-Cite XLED1, CoolLED pE-800, CoolLED pE-300 White, CoolLED pE-300 Light, CoolLED pE340 Fura |
| Leica External Filter Wheels | High speed filter wheels including (27ms switching time) |
| Leica Water Immersion Micro Dispenser | Water immersion dispenser to compensate evaporation. Ideal for screening and multi positioning experiments (compatible objectives) |
| Leica MotCorr | Motorized movement of correction ring (dedicated objectives only) |
Acquisition modules
Module name | Description | Widefield | Confocal |
| LAS X Core module | LAS X Core software, operates without the need for a dongle. | x | x |
| Multi Channel Acquisition | Allows the definition of up to 8 acquisition channels per experiment. | o | x |
| Time-Lapse | Define the duration and frequency of image capture for time lapse experiments. | o | x |
| Z-Control and Software Autofocus | Focus positioning and acquisition of 3 dimensional data sets. Includes software autofocus. | o | x |
| Mark and Find | Define multiple stage locations and revisit them as part of a time-lapse or Z stacking experiment. | o | x |
| Stitching | Create multiple regions of any shape for overview images and high resolution scans | o | x |
| Assay Editor | Perform multi well and multi chamber experiments. Create customized sample carriers. Automatically distribute regions and positions to each well/chamber. | o | o |
| Driver Non-Leica Cameras | Allows the control of any third party camera supported by LAS X. | o | o |
| Extended Depth of Field | Effectively sums up only the in focus area of each image in a three dimensional image stack and creates a single EDOF image. | o | - |
x=included; o=optional; -=not available
Advanced modules.
Module name | Description | Widefield | Confocal |
| Calcium Imaging | Module for online ratio measurement, online display of ratio graphs and ratio image | o | x |
| Live Data Mode | Interactive data recording allowing job-sequencing and online evaluation | o | o |
| Colocalization | Histogram based colocalization and area measurements | o | o |
| FRET SE | Powerful wizard for FRET acquisition and analysis | o | o |
| Environmental Control | Have full control of your experimental conditions with the LAS X Environmental Control module. Log the environmental data and monitor it during the experiment. | o | o |
| User management | Allows system administrators to give different access levels to different LAS X user groups. | o | x |
| FRAP Wizard | The step by step wizard guides users through setup, acquisition and quantification of a FRAP experiment | o | o |
| HCS A | Versatile tool set for high content screening and automated microscopy. | o | o |
| Trigger to Peripherals | Send triggers and analog signals to peripherals. Incorporate triggers into your time lapse experiments. Includes powerful trigger capabilities such as sending a trigger at a specific channel or position. | o | - |
x=included; o=optional; -=not available
Visualization and Analysis
Description | Widefield | Confocal |
| Graphic card based processing for smooth and fast motion of 3D volumes in real time | o | o |
| Wizard for 3D analysis | o | o |
| Easy and Versatile Wizard for 2D Analysis of Multi-Dimensional Data Sets | o | o |
| Fully integrated deconvolution algorithm using adaptive calculated or supplied PSF | o | o |
| Algorithms for no neighbor and nearest neighbor deconvolution | o | o |
| Multi-color restoration, channel unmixing | o | o |
| Digital overlays displayed on top of the camera image emulating fixed eyepiece graticules. | o | - |
x=included; o=optional; -=not available
Additional Functions
Module name | Description | Widefield | Confocal |
| Dye Assistant | Confocal users can select different dyes and LAS X sets up all hardware components accordingly. | - | x |
| Lambda Scan | Imaging method that acquires the emission spectrum in each image pixel using spectral detectors. | - | x |
| Lambda lambda scan | Imaging method that acquires the full excitation-emission spectrum in each image pixel using a tunable laser and spectral detectors. Allows Lambda square fluorescence mapping. | - | o |
| z-intensity compensation | Tool to compensate within a z-stack drop of fluorescence intensity that occurs deeper inside your sample with laser power and/or detector gain. | - | x |
| LightGate | Restriction of detection to a certain time window removes unwanted signal from autofluorescence or backscattering with HyD detectors. | - | o |
| FLIM | Wizard for fast and reliable setup of FLIM experiments and full control of FLIM data acquisition. | - | o |
| FCS | Wizard for fast and reliable setup of FCS and FLCS experiments and full control of F(L)CS data acquisition. | - | o |
| Electrophysiology | Package to guide you through E-Phys experiments, incl. Automated recordings, interactive data acquisition and trigger functions. | - | o |
| CRS Calculator | Automatic calculation of specific Raman wavenumbers according to the pumplaser wavelength used for exaction and vice versa. | - | o |
| CRS Spectral Scan | Automatic spectral scan covering the Raman wavenumbers range 1200-3400cm-1 | - | o |
x=included; o=optional; -=not available
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