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Technical Specifications

  Mica Widefield Mica Widefield Live Cell Mica WideFocal Mica WideFocal Live Cell

Transmitted Light Contrast

Integrated modulation contrast (IMC), automatically adjusted and Brightfield contrast in RGB or gray scale mode

x

x

x

x

Incident Fluorescence Illumination

LED

365 nm, 470 nm, 555 nm, 625 nm

x

x

x

x

FluoSync Widefield Detection

Simultaneous Detection channels

4 with FluoSyncTM fluorophore separation

x

x

x

x

Detector type

5 MP CMOS

x

x

x

x

Confocal Illumination

Laser diodes

405 nm, 488 nm, 561 nm, 638 nm

-

-

x

x

FluoSync Confocal Detection

Detector type

HyD FS

-

-

x

x

Simultaneous Detection channels

4 with FluoSyncTM fluorophore separation

-

-

x

x

Environmental Control

Live Cell Package

Temp. (to 45 °C), CO2 (0 - 10 %), humidity

-

x

-

x

Hypoxia Upgrade

Temp. (to 45 °C), O2 (0 - 10 %), CO2 (0 – 10%), humidity

-

o

-

o

Objective Packages

Included objectives

PL FLUOTAR 1.6x/0.05
PL FLUOTAR 10x/0.32

x

x

x

x

Recommended objectives

CS2 objectives: 20x, 63x W, 63x Oil

o

o

o

o

Immersion Dispension

Closed loop water dispenser

Forming and maintaining water immersion for one objective is feedback controlled and does not require any interaction

-

x

-

x

THUNDER

Methods

ICC, SVCC, LVCC

x

x

x

x

LIGHTNING

Methods

Basic, upgradeable to LIGHTNING Expert

x

x

x

x

Vibration Isolation

Anti-vibration table

passive

x

x

x

x

Microscope Focus

Autofocus

Reflection-based Adaptive Focus Control (AFC).
Image Based Autofocus for transmitted light and fluorescence images. Can be combined with AFC.

x

x

x

x

Core Functionalities

Name Description        

FluoSyncTM

FluoSyncTM detection hardware with fully integrated digital spectral hybrid unmixing for the acquisition of up to 4 labels simultaneously

x

x

x

x

OneTouch

Sets all technical excitation and detection parameters according to the experimental demands automatically or with a single click on-demand

x

x

x

x

Focusing

Keeps sample in focus throughout the experiment with a simple selection out of 3 focus strategies

x

x

x

x

3D Imaging

Allows acquisitions of 3D volumes in widefield and confocal

x

x

x

x

Mixed TL & CLSM

Combines transmitted light with confocal imaging

-

-

x

x

Mixed TL & WF

Combines transmitted light with widefield imaging

x

x

x

x

Sample Finder

Quickly and automatically generates an in-focus overview of the relevant sample areas

x

x

x

x

Navigator

Powerful package including Assay Editor, Stitching and Mark and Find license. Uses overview for navigation and for defining positions and regions in any shape. Displays all acquired images in spatial relation to all other images.

x

x

x

x

Objective Collision Prevention

Prevents the objective from colliding with a microtiter plate to protect the objective and sample

x

x

x

x

Learn & Results

Aivia-powered pixel classifier: easy to train, it generates fast and reproducible image segmentation results. The software generates beautifully visualized results with full traceability of the data points to the source in the image.

x

x

x

x

x = included, o = optional, - = not available