Mica The world’s first Microhub.
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Technical Specifications
Mica Widefield | Mica Widefield Live Cell | Mica WideFocal | Mica WideFocal Live Cell | ||
Transmitted Light Contrast | |||||
Integrated modulation contrast (IMC), automatically adjusted and Brightfield contrast in RGB or gray scale mode | x | x | x | x | |
Incident Fluorescence Illumination | |||||
LED | 365 nm, 470 nm, 555 nm, 625 nm | x | x | x | x |
FluoSync Widefield Detection | |||||
Simultaneous Detection channels | 4 with FluoSyncTM fluorophore separation | x | x | x | x |
Detector type | 5 MP CMOS | x | x | x | x |
Confocal Illumination | |||||
Laser diodes | 405 nm, 488 nm, 561 nm, 638 nm | - | - | x | x |
FluoSync Confocal Detection | |||||
Detector type | HyD FS | - | - | x | x |
Simultaneous Detection channels | 4 with FluoSyncTM fluorophore separation | - | - | x | x |
Environmental Control | |||||
Live Cell Package | Temp. (to 45 °C), CO2 (0 - 10 %), humidity | - | x | - | x |
Hypoxia Upgrade | Temp. (to 45 °C), O2 (0 - 10 %), CO2 (0 – 10%), humidity | - | o | - | o |
Objective Packages | |||||
Included objectives | PL FLUOTAR 1.6x/0.05 | x | x | x | x |
Recommended objectives | CS2 objectives: 20x, 40x W, 40x Oil, 63x W, 63x Oil | o | o | o | o |
Immersion Dispension | |||||
Closed loop water dispenser | Forming and maintaining water immersion for one objective is feedback controlled and does not require any interaction | - | x | - | x |
THUNDER | |||||
Methods | ICC, SVCC, LVCC | x | x | x | x |
LIGHTNING | |||||
Methods | Basic, upgradeable to LIGHTNING Expert | x | x | x | x |
Vibration Isolation | |||||
Anti-vibration table | passive | x | x | x | x |
Microscope Focus | |||||
Autofocus | Reflection-based Adaptive Focus Control (AFC). | x | x | x | x |
Core Functionalities | |||||
Name | Description | ||||
FluoSyncTM | FluoSyncTM detection hardware with fully integrated digital spectral hybrid unmixing for the acquisition of up to 4 labels simultaneously | x | x | x | x |
OneTouch | Sets all technical excitation and detection parameters according to the experimental demands automatically or with a single click on-demand | x | x | x | x |
Focusing | Keeps sample in focus throughout the experiment with a simple selection out of 3 focus strategies | x | x | x | x |
3D Imaging | Allows acquisitions of 3D volumes in widefield and confocal | x | x | x | x |
Mixed TL & CLSM | Combines transmitted light with confocal imaging | - | - | x | x |
Mixed TL & WF | Combines transmitted light with widefield imaging | x | x | x | x |
Sample Finder | Quickly and automatically generates an in-focus overview of the relevant sample areas | x | x | x | x |
Navigator | Powerful package including Assay Editor, Stitching and Mark and Find license. Uses overview for navigation and for defining positions and regions in any shape. Displays all acquired images in spatial relation to all other images. | x | x | x | x |
Objective Collision Prevention | Prevents the objective from colliding with a microtiter plate to protect the objective and sample | x | x | x | x |
Learn & Results | Aivia-powered pixel classifier: easy to train, it generates fast and reproducible image segmentation results. The software generates beautifully visualized results with full traceability of the data points to the source in the image. | x | x | x | x |
x = included, o = optional, - = not available