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  • 3D Biology

    3D Biology

    Improve Your 3D Cell Biology Workflow with Digital LightSheet Microscopy.The investigation of cellular spheroids with the Leica SP8 Digital LightSheet (DLS) microscope reveals meaningful results of…
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  • DLS Sample Preparation: Using U-Shaped Glass Capillaries for Sample Mounting
    Science Lab Topic: Light Sheet Microscopy

    DLS Sample Preparation: Using U-Shaped Glass Capillaries for Sample Mounting

    The TCS SP8 DLS microscope system from Leica Microsystems is an innovative concept which integrates the Light Sheet Microscopy technology into the SP8 confocal platform. Due to its unique optical…
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  • Clarifying Tissue Clearing
    Science Lab Topic: Confocal Microscopy

    Clarifying Tissue Clearing

    Biological specimens are intrinsically three dimensional; however because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to eliminate the…
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  • Clearing of Fixed Tissue: A Review from a Microscopist’s Perspective
    Science Lab Topic: Multiphoton Microscopy

    Clearing of Fixed Tissue: A Review from a Microscopist’s Perspective

    Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing interest in this…
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  • Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent
    Science Lab Topic: Super-Resolution

    Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent

    Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we report an optimized optical clearing agent for…
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  • Light Sheet Microscopy Turned  Vertically
    Science Lab Topic: Light Sheet Microscopy

    Light Sheet Microscopy Turned Vertically

    Living cells and organisms often suffer from the high light intensities used for fluorescent imaging. Light sheet microscopy reduces phototoxic effects and bleaching by illuminating a specimen in only…
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  • "The Leica Digital Light Sheet Module – a Clever Example of Thinking Out of the Box"
    Science Lab Topic: Light Sheet Microscopy

    "The Leica Digital Light Sheet Module – a Clever Example of Thinking Out of the Box"

    Bram van den Broek is a postdoctoral fellow at the Netherlands cancer institute in Amsterdam where he supports the advanced microscopy techniques in the laboratory of Kees Jalink. Working with Leica…
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  • Confocal and Digital Light Sheet Imaging
    Science Lab Topic: Light Sheet Microscopy

    Confocal and Digital Light Sheet Imaging

    Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the…
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  • Light Sheet Fluorescence Microscopy: Beyond the Flatlands
    Science Lab Topic: Light Sheet Microscopy

    Light Sheet Fluorescence Microscopy: Beyond the Flatlands

    Light Sheet Fluorescence Microscopy (LISH-M) is a true fluorescence optical sectioning technique, first described by Heinrich Siedentopf in 1902 under the name of Ultramicroscopy. Light sheet…
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  • Quantitative Imaging in Cell Biology: Light Sheet Microscopy
    Science Lab Topic: Light Sheet Microscopy

    Quantitative Imaging in Cell Biology: Light Sheet Microscopy

    This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an…
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  • Light Sheet Fluorescence Microscopy - A Review
    Science Lab Topic: Light Sheet Microscopy

    Light Sheet Fluorescence Microscopy - A Review

    Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This…
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  • Selective Plane Illumination Microscopy Techniques in Developmental Biology
    Science Lab Topic: Light Sheet Microscopy

    Selective Plane Illumination Microscopy Techniques in Developmental Biology

    Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in…
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