Get what you need and increase efficiency in your live cell imaging workflow with cell and tissue culture inverted microscopes from Leica Microsystems.
These easy-to-use microscopes allow you to configure an imaging solution for your needs with flexible condenser options and digital imaging documentation features; creating a solution that is just right for your lab.
Leica cell culture microscope systems feature
- Easy-to-use operation that requires minimal training and maintenance so you can fully concentrate on your research
- Cool, color-safe LED illumination for constant color temperature through all stages of intensity
- Easy fluorescence (optional) to easily visualize your fluorescent markers
- HD imaging (optional) - connect the HD camera directly to a monitor or PC; providing high quality publication images
- Flexible working distance up to 80 mm for accommodating slides, petri dishes, multi-well dishes, and taller flasks
- Cell factory/hotel solution fits vessels up to 400mm tall
Find your Cell Culture Solution
When it comes to cell cultures there are a couple of differences between the solution´s instruments. If you are looking for a cell culture solution please answer these four quick questions.
Do you use fluorescence?
Do you require standardized results?
Do you need a camera?
Which vessels do you use?
The appearances of cells
Lab grown animal cells can be distinguished due to several criteria:
- Their morphology is easy to identify in the microscope. Whereas fibroblast-like cells have a bi- or multipolar, elongated shape, epithelial-like cells show a polygonal outline. In contrast to the upper two, lymphoblast-like cells don’t grow attached to a surface but in suspension.
- The type of a cell can be subdivided into immortalized cells, primary cells and stem cells.
- The cell organization can range from simple 2D mono-culture through 2D co-culture, to 3D spheroids and organoids
African green monkey
A few examples of cell lines used in cell culture.
How to grow cells
Animal cells are cultured in all kinds of different vessels, ranging from tiny microfluidic devices used for basic research, through 96-well plates for screening, to cell culture flasks and cell factories for large scale pharmaceutical production.
Due to their disposable use, the majority of containers is made of plastic. Others are specifically adopted to microscopy applications and therefore have a glass bottom.
The medium for animal cell culture contains
- an energy source
- amino acids
- and salts
Moreover it includes a buffer system plus a pH indicator to check a balanced pH.
What is the daily work?
Since the ingredients in the culture medium are consumed by the cells, it is necessary to renew it regularly. On this occasion cell cultures should undergo a visual check for confluency, healthiness and to detect potential microbiological contamination.
A characteristic of immortalized cell lines is their indefinite growth. That’s why they have to be split up once in a while (Passaging) and transferred to separate culture vessels.
Commonly, cultured cells are genetically modified before using them for an experiment. With the help of transfection, researchers e.g. add fluorescent markers to their protein of interest in order to visualize it with a microscope.
What tool do I need?
To manage the daily work of a cell culture lab a microscope is needed. This microscope has to have an inverse configuration. Such an inverted microscope features the objective below and the condenser above the specimen, enabling a near enough proximity of the objective to the cells and a large working distance above.
Due to the very low intrinsic contrast of animal cells, a cell culture microscope has to deliver contrast methods such as phase contrast. DIC (Differential Interference Contrast) doesn’t help here, since it can’t be applied together with the plastic vessels used in cell culture. A very good alternative for DIC is IMC (Integrated Modulation Contrast), which works with plastic containers and in addition doesn’t need special objectives or prisms. Moreover, a cell culture microscope should be easy to handle to prevent loss of time.
Leica cell culture microscopes offer you the ease of use and the flexibility in contrast methods you need for your individual purpose.
What tool do I need?
A very common cell biological approach is to transfect cells with fluorescent markers for subsequent investigation with a research microscope. If you are dealing with fluorescent proteins, also your cell culture microscope needs a fluorescence option e.g. to control transfection efficiency.
For a meaningful documentation and standardization, the microscope should feature a digital camera and ideally the ability to record and digest the acquired data.
As space is an issue in cell culture labs, a cell culture microscope shouldn’t be too big e.g. to fit under a hood. Moreover, recent trends demand microscopes which are small and robust enough to be used even inside an incubator.
Leica DM IL LED
PH: 5x to 63x
IMC: 10x, 20x, 32x, 40x
40 mm, 80 mm
+ (free choice)
10x, 20x, 40x
40 mm, 50 mm, 80 mm
Microscopes dedicated to cell culture lab work.
Leica Cell Culture Products
Filter by Area of Application
12 MP stand-alone microscope camera to capture, document, and share images in seconds
The THUNDER Imager EM Cryo CLEM enables precise identification of cellular structures and smooth, secure transfer of coordinates, images, and samples through your correlative workflow.
Smart Cell Imager
With the automated EM TP Tissue Processor, ultrastructures of your tissue samples can be precisely prepared every time.
Ultramicrotome for array tomography
For routine to live cell research
High vacuum sputter, carbon thread and e-beam coater
High-End EM Sample Preparation Freeze Fracture System
Ultramicrotome for Perfect Sectioning at Room Temperature and Cryo
Entry level inverted microscope
Inverted Laboratory Microscope with LED Illumination
The production of biomaterials, for example proteins, vaccines or antibodies requires cell culture in large vessels.
To cope with this requirements, such a cell culture microscope has to feature a large working distance and field of view. Furthermore it needs a high stability to hold large vessels or even stacks of vessels securely.
The Leica DM IL LED can be configured with an extra-long transmitted light illumination arm, giving you the free working distance you need for cell culture vessels up to a height of 40 cm.
Cell culture is dynamic. Like any other biological system, cell growth and behavior are sometimes not easy to predict. That is why a permanent surveillance can be advantageous compared to cell culture checks at single time points.
This is where microscopes join the game which can be placed in the incubator and are able to monitor the cells around the clock. With this feature, a cell culture can be checked e.g. for confluency from any place in the world at any time.
Live Webinar "Improvement of imaging techniques to understand organelle membrane cell dynamics"
19th November 2020 | Join Simona Paladino, from Naples University, in a live webcast with Nature to learn more about improvement of imaging techniques…
Webinar - Dynamic Molecular Imaging Insights for Structural Biology
Join a Conversation about Structural Biology & Functional Imaging
Wednesday 16th September
London 16:00 | Berlin 17:00 | Dubai 19:00
How can PAULA help streamline the workflow for my wound healing assay?
Application for wound healing assays
New LMD Software 8.2.1 for Laser Microdissection
Leica Microsystems has now released version 8.2.1 of the LMS software for Laser Microdissection microscopes. Laser Microdissection is used to dissect…
Confluency Check with PAULA Cell Imager
Monitor your cell culture around the clock with PAULA and know the perfect time-point for your experiment
UC San Diego and Leica Microsystems Establish Center of Excellence in Microscopy
The new Center will support a combined mission to educate the next generation of scientists and drive new scientific discoveries
Winners of the Human Protein Atlas Image Classification Announced
Kaggle Competition Prize Money Sponsored by Leica Microsystems
Leica Microsystems Receives Prestigious R&D 100 Award
With SP8 FALCON, Leica Microsystems combines confocal and Fluorescence Lifetime IMaging (FLIM) capabilities optimizing the understanding of the sub…
New Kaggle Competition: Deep Learning Analysis of Confocal Images
Human Protein Atlas 2018 Image Challenge Sponsored by Leica Microsystems: Determine Protein Localization in Cells
Leica Microsystems Opens First Ever Imaging Center in San Francisco
Oyster Point Marina is now home to a cutting-edge life science research imaging center, offering access to state-of-the-art microscopy systems for…
13. November 2020 Studying Human Brain Development and Disease
Neural spheroids created from human induced pluripotent stem cells (iPSCs) provide effective and novel tools for studying brain development, as well…
11. November 2020 Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry
Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now…
28. September 2020 Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using…
24. September 2020 Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on…
13. July 2020 Microscopy in Virology
The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in…
08. May 2020 Computational Clearing - Enhance 3D Specimen Imaging
This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and…
25. March 2020 THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows
This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in…
20. February 2020 Multiphoton Microscopy Publication List
Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of…
19. August 2019 Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex
Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer…
18. March 2019 How can PAULA help streamline the workflow for my wound healing assay?
The wound healing assay, often used as a synonym for scratch assay or migration assay, is an important tool for the evaluation and measurement of cell…