Biowissenschaften
Biowissenschaften
Hier können Sie Ihr Wissen, Ihre Forschungsfähigkeiten und Ihre praktischen Anwendungen der Mikroskopie in verschiedenen wissenschaftlichen Bereichen erweitern. Erfahren Sie, wie Sie präzise Visualisierung, Bildinterpretation und Forschungsfortschritte erzielen können. Hier finden Sie aufschlussreiche Informationen über fortgeschrittene Mikroskopie, Bildgebungsverfahren, Probenvorbereitung und Bildanalyse. Zu den behandelten Themen gehören Zellbiologie, Neurowissenschaften und Krebsforschung mit Schwerpunkt auf modernsten Anwendungen und Innovationen.
Was ist FRET mit FLIM (FLIM-FRET)?
Der Beitrag erläutert die FLIM-FRET-Methode, die Resonanzenergietransfer und Fluoreszenz-Lebensdauer-Imaging zur Untersuchung von Protein-Protein Wechselwirkungen kombiniert.
Ratiometric Imaging and Analysis of Ion Concentration in Cells
Many cellular functions depend on the dynamic balance of ions, electric potentials, and pH between the cytosol and surrounding extracellular space. Changes in these values affect cellular function.…
High-Pressure Freezing Protocols for Ultrastructural 3D EM
High pressure freezing (HPF) can help preserve hydrated cells and tissues close to their biological state at the moment of immobilization, supporting more reliable ultrastructural interpretation than…
Ultramicrotome UC Enuity in Practice: Stable 15 nm Sections at ZFE
After using the UCT and UC6 ultramicrotomes, Claudia Mayrhofer calls UC Enuity a leap in stability—so robust that vibrations and temperature shifts don’t spoil sections, even with multiple users. Auto…
Eliminating Electrostatic Interference in Laser Microdissection
Electrostatic charge in laser microdissection (LMD) causes two critical failures: samples stick to charged surfaces and are lost, or samples fly into adjacent wells and cause cross-contamination. We…
History, Developments and Trends of Microscopy in Cancer Research
Cancer is a global disease, with 18 million new cases diagnosed and 10 million cancer-related deaths worldwide in 2020. This burden is set to increase, with a projected increase in cases of ~55% by…
Overview of Fluorescent Dyes in terms of Applications and Properties
An introduction to commonly used fluorescent dyes and an overview of their characteristics are given in this article. Fluorescence microscopy is used for the study of specific cellular components with…
Researchers Insights: Microscopy in Cancer Research
Discover how imaging techniques are driving cancer research forward. In this issue, we present comprehensive multimodal studies using microscopy, as well as new directions in intraoperative cancer…
A Guide to Fluorescence Microscopy
Fluorescence microscopy uses the ability of fluorophores, dyes, or fluorescent proteins to emit light of a specific wavelength after being excited with light of a shorter wavelength. Biomolecules can…
