Leica EM VCT500
Sample Preparation for Electron Microscopy
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Leica EM VCT500 Vacuum Cryo Transfer System
- Imaging bacteria inside their host by cryo-focused ion beam milling and electron cryotomography.
Curr Opin Microbiol. 2017 Dec 29;43:62-68. doi: 10.1016/j.mib.2017.12.006.
Medeiros JM, Böck D, Pilhofer M.
https://www.ncbi.nlm.nih.gov/pubmed/29291450 - In situ architecture, function, and evolution of a contractile injection system.
Science. 2017 Aug 18;357(6352):713-717. doi: 10.1126/science.aan7904.
Böck D, Medeiros JM, Tsao HF, Penz T, Weiss GL, Aistleitner K, Horn M, Pilhofer M.
https://www.ncbi.nlm.nih.gov/pubmed/28818949 - In-vacuum long-wavelength macromolecular crystallography
Armin Wagner, Ramona Duman, Keith Hendersona and Vitaliy Mykhaylyka
http://journals.iucr.org/d/issues/2016/03/00/ba5241/index.html - He J, Hsieh C, Wu Y, Schmelzer T, Wang P, Lin Y, Marko M, Sui H.
Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM
J Struct Biol. 2017 Aug; 199(2): 114–119.
https://www.sciencedirect.com/science/article/pii/S1047847717300916 - A Schertel, N Saidero, HM Han, T Ruhwedel, M Laue, M Grabenbauer, W Möbius.
Cryo FIB-SEM: Volume imaging of cellular ultrastructure in native frozen specimens
Journal of Structural Biology 184 (2013) 355–360
https://www.sciencedirect.com/science/article/pii/S1047847713002657 - N Vidavsky, A Akiva, I Kaplan-Ashiri, K Rechav, L Addadi, S Weiner, A Schertel
Cryo-FIB-SEM serial milling and block face imaging: Large volume structural analysis of biological tissues preserved close to their native state
J Struct Biol. 2016 Dec;196(3):487-495
https://www.sciencedirect.com/science/article/pii/S1047847716302052 - De Samber B, Meul E, Laforce B, De Paepe B, Smet J, De Bruyne M, De Rycke R, Bohic S, Cloetens P, Van Coster R, Vandenabeele P, Vanden Berghe T.
Nanoscopic X-ray fluorescence imaging and quantification of intracellular key-elements in cryofrozen Friedreich's ataxia fibroblasts.
PLoS One. 2018 Jan 17;13(1)
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0190495 - Jantschke A, Pinkas I, Hirsch A, Elad N, Schertel A, Addadi L, Weiner S.
Anhydrous β-guanine crystals in a marine dinoflagellate: Structure and suggested function.
J Struct Biol. 2019 Jul 1;207(1):12-20.
https://www.sciencedirect.com/science/article/pii/S1047847719300759 - M Bennet, L Bertinetti, RK Neely, A Schertel, A Körnig, C Flors, FD Müller, D Schüler, S Klumppf and D Faivre
Biologically controlled synthesis and assembly of magnetite nanoparticles†
Faraday Discuss. 2015;181:71-83.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672721/ - Medeiros JM, Böck D, Weiss GL, Kooger R, Wepf RA, Pilhofer M.
Robust workflow and instrumentation for cryo-focused ion beam milling of samples for electron cryotomography.
Ultramicroscopy. 2018 Jul;190:1-11.
https://www.sciencedirect.com/science/article/abs/pii/S0304399117304230?via%3Dihub - Weber MS, Wojtynek M, Medalia O.
Cellular and Structural Studies of Eukaryotic Cells by Cryo-Electron Tomography.
Cells. 2019 Jan 16;8(1).
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356268/ - Weiss GL, Kieninger AK, Maldener I, Forchhammer K, Pilhofer M.
Structure and Function of a Bacterial Gap Junction Analog.
Cell. 2019 Jul 11;178(2):374-384.
https://www.sciencedirect.com/science/article/pii/S0092867419306269