Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation
Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in…
Zebrafish Brain - Whole Organ Imaging at High Resolution
Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the vertebrate central nervous system. To image a complete…
What is a Spectral Detector (SP Detector)?
The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP detector splits light into up to 5 spectral bands.…
What is a Resonant Scanner?
A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal and multiphoton laser scanning). High acquisition…
Improve 3D Cell Biology Workflow with Light Sheet Microscopy
Understanding the sub-cellular mechanisms in carcinogenesis is of crucial importance for cancer treatment. Popular cellular models comprise cancer cells grown as monolayers. But this approach…
What is a Field-of-View Scanner?
A field-of-view scanner is an assembly of galvanometric scanning mirrors used in single-point confocal microscopes that offer the correct optical recording of large field sizes. The field-of-view…
Resolved Field Number (RFN)
The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is observable through the eyepieces. Although, we cannot…
What is a Tandem Scanner?
A Tandem Scanner is an assembly of two different types of scanning together in one system for true confocal point scanning. The Tandem Scanner consists of a three-mirror scanning base with the…
![3D glomeruli in a portion of an ECi-cleared kidney scanned by light sheet microscopy. Courtesy of Prof. Norbert Gretz, Medical Faculty Mannheim, University of Heidelberg [1].](/fileadmin/_processed_/d/d/csm_DLS-Sample-Preparation-Intr_915e0fd7c2.jpg "3D glomeruli in a portion of an ECi-cleared kidney scanned by light sheet microscopy. Courtesy of Prof. Norbert Gretz, Medical Faculty Mannheim, University of Heidelberg [1].")
Using Mounting Frames for Light Sheet Sample Preparation
Sample handling is an important topic in the context of Light Sheet Microscopy. The TCS SP8 DLS integrates Light Sheet technology into an inverted confocal platform and can hence make use of general…
