High pressure freezing with the possibility of light and electrical stimulation
High Pressure Freezer Leica EM ICE
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Science Lab Topic:
EM Sample Preparation
How to Keep Your Samples Under Physiological Conditions
Leica Microsystems’ Nano workflow combines dynamic data with the best possible sample fixation (EM ICE 70) by high pressure freezing. However, good sample preservation won’t help if your cells are…
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Science Lab Topic:
EM Sample Preparation
Leica Nano Workflow Webinar
With the Leica Nano Workflow, searching for the needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…
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Science Lab Topic:
EM Sample Preparation
Putting Dynamic Live Cell Data into the Ultrastructural Context
With the Leica Nano Workflow, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…
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Science Lab Topic:
EM Sample Preparation
Exploring the Structure and Life Cycle of Viruses
The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…
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Science Lab Topic:
EM Sample Preparation
Fast, high-quality vitrification with the EM ICE High Pressure Freezer
The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…
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Science Lab Topic:
EM Sample Preparation
Investigating Synapses in Brain Slices with Enhanced Functional Electron Microscopy
A fundamental question of neuroscience is: what is the relationship between structural and functional properties of synapses? Over the last few decades, electrophysiology has shed light on synaptic…
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Science Lab Topic:
EM Sample Preparation
High-pressure freezing: Revealing functional mechanisms of synaptic transmission
Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…
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Science Lab Topic:
EM Sample Preparation
Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
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Science Lab Topic:
EM Sample Preparation
Study synaptic dynamics with millisecond precision in neurobiology
What if you could dissect the synaptic dynamics with millisecond precision? To resolve controversial questions in neurobiology employing new techniques for visualizing cellular activity is essential.…
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Science Lab Topic:
EM Sample Preparation
Ultrastructural Preservation and Improved Visualization of Membranes in Primary Bovine Chromaffin Cells
Intracellular dynamic events, such as protein trafficking, recycling, and degradation as well as signal transduction, to name a few, can be studied using live-cell imaging techniques. However,…
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Science Lab Topic:
EM Sample Preparation
Expert Knowledge on High Pressure Freezing and Freeze Fracturing in the Cryo SEM Workflow
Get an insight in the working methods of the laboratory and learn about the advantages of Cryo SEM investigation in EM Sample Preparation. Find out how high pressure freezing, freeze fracturing and…
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Science Lab Topic:
EM Sample Preparation
Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation
Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in…
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Science Lab Topic:
EM Sample Preparation
Cryo-SEM analysis of UV light stimulated sun screen lotion
The EM ICE is the only available high-pressure freezer combining light stimulation with fast cryo fixation, a module mostly used for optogenetic approaches in neurobiology studies. However, this…
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Science Lab Topic:
EM Sample Preparation
Superior Ultrastructural Preservation and Structural Contrast in Drosophila Tissue
Optimal structural preservation of tissue can only be obtained by high pressure freezing. However, preparing the samples in optimal conditions is challenging. This article explains in detail how to…
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Science Lab Topic:
EM Sample Preparation
Expanding the Limits of Electron Microscopy Sample Preparation
Capturing the intricate changes in fine structure or in cell dynamics with conventional cryo solutions can be challenging sometimes. Leica Microsystems has developed a new cryo platform, the Leica EM…
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Science Lab Topic:
EM Sample Preparation
Revealing Cellular Dynamics with Millisecond Precision
What if you can dissect the cellular dynamics with millisecond precision?
What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy?
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Science Lab Topic:
EM Sample Preparation
Interview with Dr. Shigeki Watanabe on Research in Synaptic Membrane Dynamics
Dr. Shigeki Watanabe, principle investigator of the department of Cell Biology at the Johns Hopkins University School of Medicine in Baltimore, held a workshop in Zürich, Switzerland on methods to…
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Science Lab Topic:
EM Sample Preparation
Visualization of Membrane Dynamics with Millisecond Temporal Resolution
Application Note for Leica EM ICE, Leica EM AFS2 - Electrical stimulation of neurons combined with high-pressure freezing allows physiological activation of synaptic activity and precise control over…
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Science Lab Topic:
EM Sample Preparation
Structural Study of C. elegans
Application Note for Leica EM ICE, Leica EM AFS2 - Wildtype L4 stage C. elegans (N2 strain) were placed in the 100 μm deep side of Lecithin-coated (see detailed protocol*) type A 3 mm Cu/Au carriers…
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Science Lab Topic:
EM Sample Preparation
Cultured Rat Hippocampal Neurons
Application Note for Leica EM ICE - Rat Hippocampal neurons, cultured on 50 μm thick Aclar (Aclar embedding film, EMS) for 19 days, were frozen in the 100 μm deep side of lecithin coated (detailed…
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Science Lab Topic:
EM Sample Preparation
Symmetric Synapse - Clathrin Coated Endocytosis Pit in the Postsynaptic Dendrite
Application Note for Leica EM ICE - WT hippocampal neurons were plated at a density of 80,000 cells/cm2 on 6 mm sapphire disks for 14 days. Sample were frozen using a high-pressure freezer (Leica EM…
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Science Lab Topic:
EM Sample Preparation
High Pressure Freezing with Light Stimulation
Sun screen lotion was carefully filled in the 100 μm incision of a 3 mm copper/gold plated flat carrier and covered with 3 mm sapphire disk. The sun screen lotion sample was then high pressure frozen…
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Science Lab Topic:
CLEM
CLEM: Combining the Strengths of Light and Electron Microscopy
In recent years light microscopy studies have been dominated by live cell imaging while electron microscopy has been used for high-resolution studies. Latterly, there has been increasing interest in…
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Fields of Application
Virology
Do your research interests focus on viral infection and disease? Find out how you can gain insights into virology with solutions for imaging and sample preparation from Leica Microsystems.
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Model Organisms in Research
A model organism is a species used by researchers to study specific biological processes. They have similar genetic characteristics to humans and are commonly used in research areas such as genetics,…
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