Differential Interference Contrast (DIC) Microscopy
This article demonstrates how differential interference contrast (DIC) can be actually better than brightfield illumination when using microscopy to image unstained biological specimens.
Going Beyond Deconvolution
Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
Phase Contrast and Microscopy
This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.
Immersion Objectives
How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.
Microscope Resolution: Concepts, Factors and Calculation
This article explains in simple terms microscope resolution concepts, like the Airy disc, Abbe diffraction limit, Rayleigh criterion, and full width half max (FWHM). It also discusses the history.
Perform Microscopy Analysis for Pathology Ergonomically and Efficiently
The main performance features of a microscope which are critical for rapid, ergonomic, and precise microscopic analysis of pathology specimens are described in this article. Microscopic analysis of…
Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
How to Sanitize a Microscope
Due to the current coronavirus pandemic, there are a lot of questions regarding decontamination methods of microscopes for safe usage. This informative article summarizes general decontamination…
Resolved Field Number (RFN)
The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is observable through the eyepieces. Although, we cannot…
