Science Lab

Bem-vindo ao portal de conhecimento da Leica Microsystems. Você encontrará pesquisas científicas e material didático sobre o tema microscopia. O portal oferece suporte a iniciantes, profissionais experientes e cientistas em seus trabalhos e experimentos diários. Explore tutoriais interativos e notas de aplicação, descubra os fundamentos da microscopia, bem como as tecnologias de ponta. Faça parte da comunidade do Science Lab e compartilhe sua experiência.

Spherulitic crystals of hippuric acid, a carboxylic acid found in urine, imaged with crossed polarizers showing so-called Maltese crosses. Spherulitic structures are formed by radial crystal growth starting from a central crystal nucleus. Image recorded with a DM4 P microscope using transmitted light, 20x Plan Fluotar objective, and polarizers.

A Guide to Polarized Light Microscopy

Polarized light microscopy (POL) enhances contrast in birefringent materials and is used in geology, biology, and materials science to study minerals, crystals, fibers, and plant cell walls.

Molecular structure of the green fluorescent protein (GFP)

Introduction to Fluorescent Proteins

Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.

Fluorescence microscope image of a life-science specimen

An Introduction to Fluorescence

This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.

Image of MDCK (Madin-Darby canine kidney) cells taken with phase contrast.

Phase Contrast and Microscopy

This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.

Microscópios de campo escuro

O método de contraste de campo escuro explora a difração ou a dispersão de luz a partir de estruturas de uma amostra biológica ou as características não uniformes de uma amostra de material.

A Guide to Phase Contrast

A phase contrast light microscope offers a way to view the structures of many types of biological specimens in greater contrast without the need of stains.

The Fundamentals and History of Fluorescence and Quantum Dots

At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and…

Introduction to Widefield Microscopy

This article gives an introduction to widefield microscopy, one of the most basic and commonly used microscopy techniques. It also shows the basic differences between widefield and confocal…

Basic Principles of Luminescence

There are a lot of light-emitting processes occurring in nature. Luminescence is an umbrella term for those kinds of events where light emission is not the result of high temperatures. This article…