Biowissenschaften

Biowissenschaften

Biowissenschaften

Hier können Sie Ihr Wissen, Ihre Forschungsfähigkeiten und Ihre praktischen Anwendungen der Mikroskopie in verschiedenen wissenschaftlichen Bereichen erweitern. Erfahren Sie, wie Sie präzise Visualisierung, Bildinterpretation und Forschungsfortschritte erzielen können. Hier finden Sie aufschlussreiche Informationen über fortgeschrittene Mikroskopie, Bildgebungsverfahren, Probenvorbereitung und Bildanalyse. Zu den behandelten Themen gehören Zellbiologie, Neurowissenschaften und Krebsforschung mit Schwerpunkt auf modernsten Anwendungen und Innovationen.

FluoSync - a Fast & Gentle Method for Unmixing Multicolor Images

In this white paper, we focus on a fast and reliable method for obtaining high-quality multiplex images in fluorescence microscopy. FluoSync combines an existing method for hybrid unmixing with…
Combining spectrally resolved detection and fluorescence lifetime multiplexing

Live-Cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores

On-demand video: Imaging more subcellular targets by using fluorescence lifetime multiplexing combined with spectrally resolved detection.
Donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).

Was ist FRET mit FLIM (FLIM-FRET)?

Der Beitrag erläutert die FLIM-FRET-Methode, die Resonanzenergietransfer und Fluoreszenz-Lebensdauer-Imaging zur Untersuchung von Protein-Protein Wechselwirkungen kombiniert.

Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches

The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.
Living HeLa cells stained with WGA-488 (yellow), SPY-Actin (cyan), and SiR-Tubulin (magenta). Instant Computational Clearing (ICC) was applied.

How to Perform Dynamic Multicolor Time-Lapse Imaging

Live-cell imaging sheds light on diverse cellular events. As many of these events have fast dynamics, the microscope imaging system must be fast enough to record every detail. One major advantage of…
Spectral separation of 11 fluorophores coupled to polystyrene beads on a STELLARIS confocal system.

Multiplexing through Spectral Separation of 11 Colors

Fluorescence microscopy is a fundamental tool for life science research that has evolved and matured together with the development of multicolor labeling strategies in cells tissues and model…
Image of murine-brain tissue showing a region removed with UV laser microdissection.

RNA Quality after Different Tissue Sample Preparation

The influence of sample preparation and ultraviolet (UV) laser microdissection (UV LMD) on the quality of RNA from murine-brain tissue cryo-sections is described in this article. To obtain good…
Projection of a confocal z-stack. Sum159 cells, human breast cancer cells kindly provided by Ievgeniia Zagoriy, Mahamid Group, EMBL Heidelberg, Germany. Blue–Hoechst - indicates nuclei, Green–MitoTracker mitochondria, and red–Bodipy - lipid droplets

New Imaging Tools for Cryo-Light Microscopy

New cryo-light microscopy techniques like LIGHTNING and TauSense fluorescence lifetime-based tools reveal structures for cryo-electron microscopy.
兔舌横向组织切片。5000 万像素图像(2326 微米 x 1739 微米),由14 × 18个图块拼接而成。荧光寿命提供了额外的对比度,使组织染色中的不同结构得以区分。

Leitfaden zur Fluoreszenzlebensdauer-Imaging-Mikroskopie (FLIM)

Die Fluoreszenzlebensdauer ist ein Maß dafür, wie lange ein Fluorophor im Durchschnitt in seinem angeregten Zustand verbleibt, bevor er durch Aussendung eines Fluoreszenzphotons in den Grundzustand…
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