Science Lab

Bem-vindo ao portal de conhecimento da Leica Microsystems. Você encontrará pesquisas científicas e material didático sobre o tema microscopia. O portal oferece suporte a iniciantes, profissionais experientes e cientistas em seus trabalhos e experimentos diários. Explore tutoriais interativos e notas de aplicação, descubra os fundamentos da microscopia, bem como as tecnologias de ponta. Faça parte da comunidade do Science Lab e compartilhe sua experiência.

C. elegans adult hermaphrodite gonades acquired using THUNDER Imager. Staining: blue - DAPI (nucleus), green - SP56 (sperm), red - RME-2 (oocyte), magenta - PGL-1 (RNA + protein granules). Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany.

Life Science Research: Which Microscope Camera is Right for You?

Deciding which microscope camera best fits your experimental needs can be daunting. This guide presents the key factors to consider when selecting the right camera for your life science research.

The principle of the FusionOptics technology: Of the two separate beam paths (1), one provides depth of field (2) and the other high resolution (3). In the brain, the two images of the sample are merged into a single, optimal 3D image (4).

What is the FusionOptics Technology?

Leica stereo microscopes with FusionOptics provide optimal 3D perception. The brain merges two images, one with large depth of field and the other with high resolution, into one 3D image.

Fluorescence microscope image of a life-science specimen

An Introduction to Fluorescence

This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.

Spirogyra algae (Conjugation), Transmitted Light Differential Interference Contrast.

Differential Interference Contrast (DIC) Microscopy

This article demonstrates how differential interference contrast (DIC) can be actually better than brightfield illumination when using microscopy to image unstained biological specimens.

Going Beyond Deconvolution

Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…

Image of MDCK (Madin-Darby canine kidney) cells taken with phase contrast.

Phase Contrast and Microscopy

This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.

Immersion Objectives

How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.

Intensity distribution (arbitrary color coding) of an image of two points where the distance between them corresponds to the Rayleigh criterion.

Microscope Resolution: Concepts, Factors and Calculation

This article explains in simple terms microscope resolution concepts, like the Airy disc, Abbe diffraction limit, Rayleigh criterion, and full width half max (FWHM). It also discusses the history.

Perform Microscopy Analysis for Pathology Ergonomically and Efficiently

The main performance features of a microscope which are critical for rapid, ergonomic, and precise microscopic analysis of pathology specimens are described in this article. Microscopic analysis of…