Fast, High Acuity Imaging and AI-assisted Analysis

June 01, 2022

Fast, High Acuity Imaging and AI-assisted Analysis

Clear imaging of whole-mounted mouse retina with THUNDER and Aivia

Mouse whole-mount retina. Image courtesy of the Experimental Ophthalmology Group, University of Murcia, Spain. Mouse_whole-mount_retina_Thundered.jpg

In this article, we demonstrate how imaging the whole-mounted mouse retina using THUNDER can dramatically boost the speed and quality of data collection. Compared to conventional widefield microscopy, THUNDER imaging provides sharper images and allows for rapid segmentation without out-of-focus blur or haze. Combined with Aivia, this allows high-powered, efficient analysis of images, with high quality and reproducible results between samples.

The challenges with out-of-focus blur and long acquisition times

In conventional widefield imaging, a common issue is out-of-focus blur, especially when imaging thick sections or whole-mounted samples like the retina. Blur is caused by illumination of the whole sample, particularly the areas above and below the image plane. This causes poor contrast of fluorophore channels, masks image information, and reduces the ability for clear and reproducible analysis. Confocal point scanners allow optical sectioning and are the benchmark for superior image quality and high-contrast fluorescence images, even for multicolour data acquisition. However, with confocal point scanners, acquiring image sequences of large samples might be time consuming depending on the scanning speed.

Together, low-throughput acquisition and blur can hamper a scientist’s ability to quantify cellular and molecular features of tissue like the retina. For this type of research, it’s important to have a solution capable of capturing images with high acuity and fast screening of the retina, so 3D reconstruction can be performed.

Key Learnings

High-acuity imaging of whole-mounted retina samples using THUNDER
Powerful image analysis of THUNDERed images with Aivia
Better quality and reproducible analysis of thick tissues like the retina

Download the application note to learn more about fast, high acuity imaging and AI-assisted analysis of retinal cell damage

Register to download

First Name *

Last Name *

Email *

Company/Institution *

Zip *

Country/Region *

Ask your question

Are you planning on making a purchase in the next 12 months? *

Yes

No

I am interested in receiving information about products and services, virtual and in-person events such as workshops and webinars and educational content from Leica Microsystems GmbH, their representatives and other Danaher companies.
I understand that I may be contacted by phone or email and consent to such communication. *

Yes

No

By clicking on the SUBMIT button, I confirm that I have reviewed and agree with Leica Microsystems GmbH Terms of Use and their Privacy Policy. I also understand my privacy choices as they pertain to my personal data, as detailed in the aforementioned Privacy Policy under ‘’Your Privacy Choices’’.

Related Pages

Microscope Imaging Software

Microscope imaging software from Leica Microsystems combines microscope, digital camera and…

Visit related page
THUNDER Imaging Systems

To answer important scientific questions, THUNDER Imaging Systems enable you to obtain a clear view…

Visit related page