How to Keep Your Samples Under Physiological Conditions
The Coral Life workflow combines dynamic data with the best possible sample fixation by high pressure freezing. However, good sample preservation won’t help if your cells are stressed by temperature…
Putting Dynamic Live Cell Data into the Ultrastructural Context
With workflow Coral Life, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the right…
Fast, High-quality Vitrification with the EM ICE High Pressure Freezer
The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…
Investigating Synapses in Brain Slices with Enhanced Functional Electron Microscopy
A fundamental question of neuroscience is: what is the relationship between structural and functional properties of synapses? Over the last few decades, electrophysiology has shed light on synaptic…
Ion Beam Milling Guide: Enhancing Surface Quality for High-Resolution Imaging and Analysis
In this article you can learn how to optimize the preparation quality of your samples by using the ion beam etching method with the EM TIC 3X ion beam milling machine. A short introduction of the…
スパッタコーティング & 凍結割断ソリューション
室温で低真空スパッタコーティング機器によるコーティングから、高真空、さらに極低温化でのコーティングまで、ライカのコーティングソリューションは非常に幅広いニーズに対応しています。
High-pressure freezing: Revealing functional mechanisms of synaptic transmission
Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…
Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
Studying the Microstructure of Natural Polymers in Fine Detail
The potential of cryogenic broad ion beam milling used in combination with scanning electron microscopy (cryo-BIB-SEM) for imaging and analyzing the microstructure of cryogenically stabilized soft…
