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Optical Contrast Methods
Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the…
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Integrated Modulation Contrast (IMC)
Hoffman modulation contrast has established itself as a standard for the observation of unstained, low-contrast biological specimens. The integration of the modulator in the beam path of themodern…
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Polarization Contrast
Polarization microscopy is routinely applied in material sciences and geology to identify minerals on the basis of characteristic refraction properties and colors. In biology, polarization microscopy…
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Fluorescence in Microscopy
Fluorescence microscopy is a special form of light microscopy. It uses the ability of fluorochromes to emit light after being excited with light of a certain wavelength. Proteins of interest can be…
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Depth of Field in Microscopy
In microscopy, depth of field is often seen as an empirical parameter. In practice it is determined by the correlation between numerical aperture, resolution and magnification. For the best possible…
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Beware of "Empty" Magnification
This article explains how to avoid the phenomen of "empty magnification" in microscopy.