Leica Microsystems: Science Lab

Science Lab

Das Wissensportal von Leica Microsystems bietet Ihnen Wissens- und Lehrmaterial zu den Themen der Mikroskopie. Die Inhalte sind so konzipiert, dass sie Einsteiger, erfahrene Praktiker und Wissenschaftler gleichermaßen bei ihrem alltäglichen Vorgehen und Experimenten unterstützen. Entdecken Sie interaktive Tutorials und Anwendungsberichte, erfahren Sie mehr über die Grundlagen der Mikroskopie und High-End-Technologien - werden Sie Teil der Science Lab Community und teilen Sie Ihr Wissen!

THUNDER image of brain-capillary endothelial-like cells derived from human iPSCs (induced pluripotent stem cells) where cyan indicates nuclei and magenta tight junctions.

Rapid Check of Live Stem Cells in Cell-Culture Inserts set in Multi-Well Plates

See how efficient imaging of live iPSC stem cells within cell-culture inserts set in a multi-well plate can be done to evaluate the cells using a THUNDER Imager. Just read this article.

Immersion Objectives

How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.

Intensity distribution (arbitrary color coding) of an image of two points where the distance between them corresponds to the Rayleigh criterion.

Microscope Resolution: Concepts, Factors and Calculation

This article explains in simple terms microscope resolution concepts, like the Airy disc, Abbe diffraction limit, Rayleigh criterion, and full width half max (FWHM). It also discusses the history.

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…

Spherical aberration describes the fact that waves which pass through the centre of the lens are refracted less than the waves which pass through the edges of the curved lens.

Eyepieces, Objectives and Optical Aberrations

This article covers the components of the eyepieces and how to adjust them correctly to suit your eyes.

Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps

In this article, we will look at the history of the technique of Koehler Illumination in addition to how to adjust the components in five easy steps.

Collecting Light: The Importance of Numerical Aperture in Microscopy

Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish detail in a specimen viewed down the microscope. NA is a number without units and is related to the…

Optimization of the Interplay of Optical Components for Aberration Free Microscopy

Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to achieve appropriate resolution. However, besides…

BABB Clearing and Imaging for High Resolution Confocal Microscopy

Multipohoton microscopy experiment using Leica TCS SP8 MP and Leica 20x/0.95 NA BABB immersion objective. Understanding kidney microanatomy is key to detecting and identifying early events in kidney…