Science Lab

Das Wissensportal von Leica Microsystems bietet Ihnen Wissens- und Lehrmaterial zu den Themen der Mikroskopie. Die Inhalte sind so konzipiert, dass sie Einsteiger, erfahrene Praktiker und Wissenschaftler gleichermaßen bei ihrem alltäglichen Vorgehen und Experimenten unterstützen. Entdecken Sie interaktive Tutorials und Anwendungsberichte, erfahren Sie mehr über die Grundlagen der Mikroskopie und High-End-Technologien - werden Sie Teil der Science Lab Community und teilen Sie Ihr Wissen!

Molecular structure of the green fluorescent protein (GFP)

Introduction to Fluorescent Proteins

Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.

Differential Interference Contrast (DIC) Microscopy

This article demonstrates how differential interference contrast (DIC) can be actually better than brightfield illumination when using microscopy to image unstained biological specimens.

Image of MDCK (Madin-Darby canine kidney) cells taken with phase contrast.

Phase Contrast and Microscopy

This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.

Immersion Objectives

How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.

Precise Spatial Proteomic Information in Tissues

Despite the availability of imaging-based and mass-spectrometry-based methods for spatial proteomics, a key challenge remains connecting images with single-cell-resolution protein abundance…

Images of smooth muscle cells during wound healing. Courtesy L.S. Shankman, Ph.D., University of Virginia.

Studying Wound Healing of Smooth Muscle Cells

This article discusses how wound healing of cultured smooth muscle cells (SMCs) in multiwell plates can be reliably studied over time with less effort using a specially configured Leica inverted…

Wie Sie Gewebeproben für die Immunfluoreszenz-Mikroskopie vorbereiten

Immunfluoreszenz (IF) ist eine leistungsfähige Methode zur Visualisierung intrazellulärer Prozesse, Bedingungen und Strukturen. IF-Präparate können mit verschiedenen Mikroskopietechniken (z. B. CLSM,…

Fluorescent Dyes

A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP –…

Images from 2 time-lapse series of the same Sf21 moth larval cells illuminated with: TIRF mode. Images were acquired with the DMi8 S Infinity TIRF HP system. Courtesy of Domokos Lauko, Welch Lab, Molecular and Cell Biology, University of California, Berkeley, USA.

Studying Actin Cytoskeleton and Microfilament Assembly

This article demonstrates how actin microfilament assemblies can be clearly visualized with superior resolution at the plasma membrane of a cell using a DMi8 S Infinity TIRF High Power imaging…