Science Lab

Benvenuti nel portale delle conoscenze di Leica Microsystems. Troverete materiale didattico e di ricerca scientifica sul tema della microscopia. Il portale supporta i principianti, i professionisti esperti e gli scienziati nel loro lavoro quotidiano e negli esperimenti. Esplorate i tutorial interattivi e le note applicative, scoprite le basi della microscopia e le tecnologie di punta. Entrate a far parte della comunità di Science Lab e condividete la vostra esperienza.

Micrograph of dinoflagellate cells. Scale bar = 1 µm.

How Marine Microorganism Analysis can be Improved with High-pressure Freezing

In this application example we showcase the use of EM-Sample preparation with high pressure freezing, freeze substiturion and ultramicrotomy for marine biology focusing on ultrastructural analysis of…

How to Successfully Perform Live-Cell CLEM

The Leica Nano workflow provides a streamlined live-cell CLEM solution for getting insight bout structural changes of cellular components over time. Besides the technical handling described in the…

How to Successfully Implement Coral Life

The live-cell  CLEM workflow allows you to capture dynamic information related to a relevant biological process as it happens and put these observations into their ultrastructural context. The Leica…

HeLa Kyoto cells (HKF1, H2B-mCherry, alpha Tubulin, mEGFP). Left image: Maximum projection of a z-stack prior to ICC and LVCC. Right image: Maximum projection of a mosaic z-stack after ICC and LVCC.

How to Improve Live Cell Imaging with Coral Life

For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…

How to Keep Your Samples Under Physiological Conditions

The Coral Life workflow combines dynamic data with the best possible sample fixation by high pressure freezing. However, good sample preservation won’t help if your cells are stressed by temperature…

Capture life as it happens

With the Leica Nano Workflow, searching for the needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…

Putting Dynamic Live Cell Data into the Ultrastructural Context

With workflow Coral Life, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the right…

Exploring the Structure and Life Cycle of Viruses

The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…

Fast, High-quality Vitrification with the EM ICE High Pressure Freezer

The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…