Science Lab

Bem-vindo ao portal de conhecimento da Leica Microsystems. Você encontrará pesquisas científicas e material didático sobre o tema microscopia. O portal oferece suporte a iniciantes, profissionais experientes e cientistas em seus trabalhos e experimentos diários. Explore tutoriais interativos e notas de aplicação, descubra os fundamentos da microscopia, bem como as tecnologias de ponta. Faça parte da comunidade do Science Lab e compartilhe sua experiência.

Large field of view (FOV) 2D slices of a 1 mm diameter midbrain neural organoid stained with DAPI (blue, nuclear stain), β-tubulin (green, neuronal stain), and GFAP (red, astrocyte stain). Acquired on THUNDER Imager Cell with 10x magnification. Left: Raw widefield image, Right: THUNDER cleared image. Scale bar 400 μm. Sample courtesy of Dr Tanya Singh, University of Oxford, UK.

Fast, High-Contrast Widefield Imaging of Optically Challenging Samples

Live‑cell imaging of large, complex biological samples often requires large fields of view, sub-cellular resolution, high-sensitivity, and fast acquisition – all while maintaining low illumination…

Hunched working position without a microscope (left) and upright neutral posture achieved with the M320 dental microscope (right).

Dental Loupes vs Microscopes: Exploring Visualization Options in Dentistry

Dental professionals often ask: “Should I use dental loupes or invest in a microscope?” This article explores the key differences between dental microscopes and dental loupes, focusing on…

Low-input spatial proteomics workflow for megakaryocyte isolation from bone marrow.

Spatial Proteomics Workflow in Blood Cancer (MPNs)

Megakaryocytes play a central role in the biology of myeloproliferative neoplasms (MPNs), yet their in vivo proteomic characterization remains a major challenge due to low abundance and disrupted…

Six Features to Consider when Choosing a Dental Microscope

The dental surgical microscope has become increasingly important for high-quality and successful dental medicine, particularly in the field of endodontics. A dentist can conduct micro-invasive…

Long-term in toto imaging of FUCCI2 intestinal organoids.

Multiscale Imaging of Organoids: High Content to Light Sheet

Learn multiscale organoid imaging: fixed high content phenotyping, gentle dual view light sheet, and reproducible pipelines that turn 3D data into insights.

Ribbon diagram of a donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).

What is FRET with FLIM or as it is usually known FLIM-FRET?

Förster resonance energy transfer (FRET) is a well-established fluorescence-based technique which is used to study molecular interactions. It is useful for the analysis of protein-DNA and…

Time-lapse imaging of calcium in cells using the ratiometric calcium indicator Fura-2.

Ratiometric Imaging and Analysis of Ion Concentration in Cells

Many cellular functions depend on the dynamic balance of ions, electric potentials, and pH between the cytosol and surrounding extracellular space. Changes in these values affect cellular function.…

Proveo 8x 3D digital microscope used in ophthalmic surgery, illustrating heads‑up visualization and clinical collaboration.

4 Key Benefits of 3D Digital Microscopy in Ophthalmic Surgery

3D digital visualization is rapidly transforming ophthalmic surgery. Modern 3D surgical microscopes enable surgeons to perform procedures using high-resolution digital displays rather than traditional…

3D rendering of a mouse beta cell with mitochondria (blue), insulin SGs (orange), microtubules (red), nucleus (yellow), and plasma membrane (transparent).

High-Pressure Freezing Protocols for Ultrastructural 3D EM

High pressure freezing (HPF) can help preserve hydrated cells and tissues close to their biological state at the moment of immobilization, supporting more reliable ultrastructural interpretation than…

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