Science Lab

Benvenuti nel portale delle conoscenze di Leica Microsystems. Troverete materiale didattico e di ricerca scientifica sul tema della microscopia. Il portale supporta i principianti, i professionisti esperti e gli scienziati nel loro lavoro quotidiano e negli esperimenti. Esplorate i tutorial interattivi e le note applicative, scoprite le basi della microscopia e le tecnologie di punta. Entrate a far parte della comunità di Science Lab e condividete la vostra esperienza.

Inspection microscope image of a printed circuit board (PCB) taken with a ring light (RL) and near vertical illumination (NVI).

Microscope Illumination for Industrial Applications

Inspection microscope users can obtain information from this article which helps them choose the optimal microscope illumination or lighting system for inspection of parts or components.

Image of MDCK (Madin-Darby canine kidney) cells taken with phase contrast.

Phase Contrast and Microscopy

This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.

Tumor resection leveraging 5-ALA fluorescence. Image courtesy of Dr. Carla Reizinho.

Surgical Management of High-Grade Gliomas

Learn about the surgical management of high-grade gliomas and how to expand the extent of resection intra-operatively using tools such as 5-ALA fluorescence.

Immersion Objectives

How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.

Phase-contrast image of a MDCK-cell culture and its respective confluency measured by the Mateo TL microscope.

How to Determine Cell Confluency with a Digital Microscope

This article shows how to measure cell confluency in an easy and consistent way with Mateo TL, increasing confidence in downstream experiments.

Microscope image of cultured cells at the bottom of a dish.

How to do a Proper Cell Culture Quick Check

In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth…

Intensity distribution (arbitrary color coding) of an image of two points where the distance between them corresponds to the Rayleigh criterion.

Microscope Resolution: Concepts, Factors and Calculation

This article explains in simple terms microscope resolution concepts, like the Airy disc, Abbe diffraction limit, Rayleigh criterion, and full width half max (FWHM). It also discusses the history.

Five-color FLIM-STED with One Depletion Laser

Webinar on five-color STED with a single depletion laser and fluorescence lifetime phasor separation.

Diffuse retinal degeneration with relative macular sparing.

Ophthalmic Gene Therapy Subretinal Injection

Case study on the use of intraoperative OCT for Leber congenital amaurosis macular repair and ophthalmic gene therapy subretinal injection.