Science Lab
Science Lab
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Introduction to Widefield Microscopy
This article gives an introduction to widefield microscopy, one of the most basic and commonly used microscopy techniques. It also shows the basic differences between widefield and confocal…
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Optimization of the Interplay of Optical Components for Aberration Free Microscopy
Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to achieve appropriate resolution. However, besides…
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Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
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Infinity Optical Systems
“Infinity Optics” refers to the concept of a beam path with parallel rays between the objective and the tube lens of a microscope. Flat optical components can be brought into this “Infinity Space”…
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A Brief History of Light Microscopy
The history of microscopy begins in the Middle Ages. As far back as the 11th century, plano-convex lenses made of polished beryl were used in the Arab world as reading stones to magnify manuscripts.…
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50 Years of Image Analysis
Modern image analysis systems perform highly sophisticated image processing functions on images from an automated microscope and digital camera. 50 years ago, the first image analysis system was…
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Video Tutorial: How to Change the Bulb of a Fluorescence Lamp Housing
When applying fluorescence microscopy in biological applications, a lamp housing with mercury burner is the most common light source. This video tutorial shows how to change the bulb of a traditional…
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Video Tutorial: How to Align the Bulb of a Fluorescence Lamp Housing
The traditional light source for fluorescence excitation is a fluorescence lamp housing with mercury burner. A prerequisite for achieving bright and homogeneous excitation is the correct centering and…
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How to Clean Microscope Optics
Clean microscope optics are essential for obtaining good microscope images. If they are dirty, the microscope should be cleaned to avoid a loss of quality. If you decide to do this yourself, you…