Science Lab

Science Lab

Science Lab

Bienvenue sur le portail de connaissances de Leica Microsystems. Vous y trouverez des recherches scientifiques et du matériel didactique sur le thème de la microscopie. Le portail aide les débutants, les praticiens expérimentés et les scientifiques dans leur travail quotidien et leurs expériences. Explorez les didacticiels interactifs et les notes d'application, découvrez les bases de la microscopie ainsi que les technologies de pointe. Faites partie de la communauté Science Lab et partagez votre expertise.
Foraminifera (Ammonia confertitesta) labeled with membrane-permeable calcein, high-pressure frozen in salt water using EM ICE. The sample was cryo-planed and targeted with the M205 on the Cryo-Fluo Enuity, then transferred under cryo conditions to the Cryo-Stellaris for widefield and confocal imaging, revealing details of the staining pattern. Image courtesy: David Evans, University of Southampton.

High-Pressure Freezing for Organoids: Cryo CLEM & FIB Lift Out

Master cryo EM workflow steps for challenging 3D samples: when to choose HPF vs. plunge freezing, reproducible blotting/ice control, contamination aware transfers, Cryo CLEM 3D targeting in organoids,…
Complete camera overview of EM grid recorded with 3 channels. Inserts displaying the positions, where superresolved 3D confocal images were recorded. 3D renderings of these positions are shown in the zoomed inserts. Fluorescence channels (nuclei by Hoechst, blue; mitochondria by MitoTracker Green, green; lipid Droplets by Bodipy and Crimson Beads, red). Width of a grid square is 90 ?m, width of a grid bar is 35 ?m. Samples kindly provided by Ievgeniia Zagoriy, Mahamid-Group, EMBL Heidelberg, Germany.

From Bench to Beam: A Complete Correlative Cryo Light Microscopy Workflow

In the webinar entitled "A Multimodal Vitreous Crusade, a Cryo Correlative Workflow from Bench to Beam" a team of experts discusses the exciting world of correlative workflows for structural biology…

Tomographie Cryoélectronique

La tomographie cryoélectronique (CryoET) est utilisée pour analyser les biomolécules dans leur environnement cellulaire avec une résolution sans précédent, inférieure au nanomètre.

Workflows and Instrumentation for Cryo-electron Microscopy

Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…

Virologie

Vos recherches portent sur les infections et les maladies virales ? Découvrez comment renforcer vos connaissances en virologie grâce aux solutions d’imagerie et de préparation d’échantillons de Leica…

Improve Cryo Electron Tomography Workflow

Leica Microsystems and Thermo Fisher Scientific have collaborated to create a fully integrated cryo-tomography workflow that responds to these research needs: Reveal cellular mechanisms at…
Roland A. Fleck

Expert Knowledge on High Pressure Freezing and Freeze Fracturing in the Cryo SEM Workflow

Get an insight in the working methods of the laboratory and learn about the advantages of Cryo SEM investigation in EM Sample Preparation. Find out how high pressure freezing, freeze fracturing and…
Cryo-electron micrographs of subviral particles of a common cold virus bound to liposomes, acquired under low dose conditions at 300 kV with a CCD-camera.

Immersion Freezing for Cryo-Transmission Electron Microscopy: Applications

A well established usage case for cryo-TEM is three-dimensional reconstruction of isolated macromolecules, virus particles, or filaments. These approaches are based on averaging of repetitive…
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