Science Lab

Science Lab

Science Lab

Bienvenido al portal de conocimiento de Leica Microsystems. Aquí encontrará investigación científica y material didáctico sobre el tema de la microscopía. El portal ayuda a principiantes, profesionales experimentados y científicos por igual en su trabajo diario y en sus experimentos. Explore tutoriales interactivos y notas de aplicación, descubra los fundamentos de la microscopía, así como las tecnologías de gama alta. Forme parte de la comunidad Science Lab y comparta sus conocimientos.
Electron microscope (EM) image of a cross section of C. elegans (roundworm). Courtesy of T. Müller-Reichert, MPI-CBG, Dresden, Germany and K. McDonald, University of California, Berkeley, USA.

Brief Introduction to High-Pressure Freezing for Cryo-Fixation

Preparation of biological specimens for electron microscopy (EM) often requires cryo-fixation which does not introduce significant structural alterations of cellular constituents. A common method used…

How to Successfully Perform Live-Cell CLEM

The Leica Nano workflow provides a streamlined live-cell CLEM solution for getting insight bout structural changes of cellular components over time. Besides the technical handling described in the…
HeLa Kyoto cells (HKF1, H2B-mCherry, alpha Tubulin, mEGFP). Left image: Maximum projection of a z-stack prior to ICC and LVCC. Right image: Maximum projection of a mosaic z-stack after ICC and LVCC.

How to Improve Live Cell Imaging with Coral Life

For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…
The EM ICE Nano loading area

How to Keep Your Samples Under Physiological Conditions

The Coral Life workflow combines dynamic data with the best possible sample fixation by high pressure freezing. However, good sample preservation won’t help if your cells are stressed by temperature…

Putting Dynamic Live Cell Data into the Ultrastructural Context

With workflow Coral Life, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the right…

Fast, High-quality Vitrification with the EM ICE High Pressure Freezer

The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…

Investigating Synapses in Brain Slices with Enhanced Functional Electron Microscopy

A fundamental question of neuroscience is: what is the relationship between structural and functional properties of synapses? Over the last few decades, electrophysiology has shed light on synaptic…
EM Sample Preparation Coating, Etching and Fracturing

Soluciones de recubrimiento con pulverización catódica y crío-fractura

Leica Microsystems cubre toda la gama de necesidades de recubrimiento, desde el recubrimiento a baja temperatura ambiente hasta el crío-recubrimiento de alto vacío.

High-pressure freezing: Revealing functional mechanisms of synaptic transmission

Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…
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