Science Lab

Science Lab

Science Lab

Bienvenido al portal de conocimiento de Leica Microsystems. Aquí encontrará investigación científica y material didáctico sobre el tema de la microscopía. El portal ayuda a principiantes, profesionales experimentados y científicos por igual en su trabajo diario y en sus experimentos. Explore tutoriales interactivos y notas de aplicación, descubra los fundamentos de la microscopía, así como las tecnologías de gama alta. Forme parte de la comunidad Science Lab y comparta sus conocimientos.
Fluorescence stereo microscope image of anesthetized Mediterranean fruit flies recorded with a M205 stereo microscope.

Investigating Fruit Flies (Drosophila melanogaster)

Learn how to image and investigate Drosophila fruit fly model organisms efficiently with a microscope for developmental biology applications from this article.
Acousto-optics, sketch

Acousto Optics in True Confocal Spectral Microscope Systems

Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this…
Exchange a fluorescence bulb.

Video Tutorial: How to Change the Bulb of a Fluorescence Lamp Housing

When applying fluorescence microscopy in biological applications, a lamp housing with mercury burner is the most common light source. This video tutorial shows how to change the bulb of a traditional…
Center a fluorescence bulb.

Video Tutorial: How to Align the Bulb of a Fluorescence Lamp Housing

The traditional light source for fluorescence excitation is a fluorescence lamp housing with mercury burner. A prerequisite for achieving bright and homogeneous excitation is the correct centering and…
Sub-Femtolitre volume_Fluorescence correlation spectroscopy (FCS)

Fluorescence Correlation Spectroscopy (FCS)

Fluorescence correlation spectroscopy (FCS) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as the diffusion time, number of molecules or dark…

Image Processing for Widefield Microscopy

Fluorescence microscopy is a modern and steadily evolving tool to bring light to current cell biological questions. With the help of fluorescent proteins or dyes it is possible to make discrete…
Live cell imaging, 4 colors: Mitochondria (MitoView Green, yellow) and actin (mNeonGreen, cyan) microtubuli (SIR-tubulin, magenta), endosomes (NIR750, green). Processed with DSE and DSE powered by Aivia.

The Principles of White Light Laser Confocal Microscopy

The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime fluorescence. Furthermore, it should offer means…

Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…

Basic Principles of Luminescence

There are a lot of light-emitting processes occurring in nature. Luminescence is an umbrella term for those kinds of events where light emission is not the result of high temperatures. This article…
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