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THUNDER Imager Live Cell & 3D Assay
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Fast, High Acuity Imaging and AI-assisted Analysis
The use of state-of-the-art AI systems is pushing image analysis into a new generation. Challenges like the conflict between imaging power and sample integrity are being overcome with THUNDER’s…
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Create New Options for Live Cell Imaging
The use of state-of-the-art AI systems is pushing image analysis into a new generation. Challenges like the conflict between imaging power and sample integrity are being overcome with THUNDER’s…
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Expression of D2-Dopamine Receptors in Neurons
This article discusses how D2-dopamine receptors (D2R) in the substantia-nigra of mouse brain can be more clearly revealed with a THUNDER Imager 3D Assay using Instant Computational Clearing (ICC)…
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Investigating Pulmonary Vascular Diseases
This article describes how the cellular and molecular programs governing the formation and maintenance of lung blood vessels, as well as what goes wrong with them when pulmonary vascular diseases…
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Phosphoprotein Regulates Integrin Endosomal Trafficking
Integrins are involved in cell-cell and cell-extracellular matrix adhesion. In addition, they mediate several signaling pathways, regulating e.g. cell motility. Caliva et al. discovered, with the help…
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Visualizing retinal interactions to study eye diseases
This article shows how interactions between endothelial cells, blood vessels, microglia, and astrocytes in mouse retina can be studied efficiently with a THUNDER Imager 3D Cell Culture and Large…
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Cranial Nerve Development
This article demonstrates how fast, high-contrast imaging of mouse embryos with a THUNDER Imager 3D Cell Culture and Large Volume Computational Clearing (LVCC) enable the investigation of axonal…
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Accurately analyze fluorescent widefield images
The specificity of fluorescence microscopy allows researchers to accurately observe and analyze biological processes and structures quickly and easily, even when using thick or large samples. However,…
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Role of Mucins and Glycosylation in Dry Eye Disease
This article shows how fast, high-contrast, and sharp imaging of stratified human corneal epithelial cells with THUNDER imaging technology for dry eye disease (DED) research allows membrane ridges to…
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Skeletal Muscle Adaptation and Fibrotic Diseases
The mechanisms of how skeletal muscle adapts to fibrotic pathologies can be investigated more efficiently with fast, high-contrast imaging of C2C12 mouse myoblast cells which is described in this…
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How to Improve Live Cell Imaging with Coral Life
For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…
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Optimizing THUNDER Platform for High-Content Slide Scanning
With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging technology are tested, while user trainability and…
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Physiology Image Gallery
Physiology is about the processes and functions within a living organism. Research in physiology focuses on the activities and functions of an organism’s organs, tissues, or cells, including the…
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Monitoring Immunosuppressive Mechanisms from Infection
This application note discusses the importance of fast, sensitive, and haze-free imaging for the monitoring of immunosuppression in mouse lung epithelial tissue infected with the Puerto Rico 8…
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Neuroscience Images
Neuroscience commonly uses microscopy to study the nervous system’s function and understand neurodegenerative diseases.
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Developmental Biology Image Gallery
Developmental biology explores the development of complex organisms from the embryo to adulthood to understand in detail the origins of disease. This category of the gallery shows images about…
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“Brains-In-A-Dish” from Induced Pluripotent Stem Cells (iPSCs)
This article discusses the benefits of using the THUNDER technology for imaging inside 3D human cortical brain organoids. These organoids are derived from human induced pluripotent stem cells (iPSCs)…
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Developing Heart Pacemaker Cells from Cardiac Spheroids
During the last decade, 3D cell culture has been established as a more realistic model compared to classical 2D culture systems. Cells can develop into miniature 3D objects, so called spheroids, which…
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Putting Dynamic Live Cell Data into the Ultrastructural Context
With workflow Coral Life, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the right…
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Into the Third Dimension with "Wow Effect"- Observe Cells in 3D and Real-Time
Life is fast, especially for a cell. As a rule, cells should be examined under physiological conditions which are as close as possible to their natural environment. New technologies offer tremendous…
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Observing 3D Cell Cultures During Development
3D cell cultures, such as organoids and spheroids, give insights into cells and their interactions with their microenvironment. These 3D cell cultures are playing an increasingly important role for…
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Cytosolic Calcium Ions in Melanoma Cancer Cells
In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging is presented…
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Download The Guide to Live Cell Imaging
In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide range of important considerations to take to…
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Studying Cell Division
Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
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Influenza-host Interactions in 3D Specimens
Stefan Finke studies virus-host interactions. It turns out that the ways the virus and host interact are different when comparing results from classical laboratory monolayer cell lines and “close to…
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Finding new Scaffolds for Tissue Engineering
Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to creating in vitro disease models. Scaffold architecture…
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The Power of Pairing Adaptive Deconvolution with Computational Clearing
Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with sources of noise. In this technical brief, learn how the…
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Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics
Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell…
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Studying Autoimmune Disease
This article discusses how autoimmune diseases, like systemic lupus erythematosus (SLE), can be studied more efficiently using thick, 3D kidney tissue specimens visualized with a THUNDER Imager . SLE…
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Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…
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From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…
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Studying Human Brain Development and Disease
Neural spheroids created from human induced pluripotent stem cells (iPSCs) provide effective and novel tools for studying brain development, as well as the underlying pathological mechanisms of…
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An Introduction to Computational Clearing
Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
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Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
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How Can Immunofluorescence Aid Virology Research?
Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…
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Studying Natural Killer (NK) Cells Derived from Induced Pluripotent Stem Cells (iPSC)
The study of natural killer (NK) cells holds tremendous promise for developing novel immunotherapies. NK cells derived from induced pluripotent stem cells (iPSCs) can be used to create an easily…
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Getting Sharper 3D Images of Thick Biological Specimens with Widefield Microscopy
Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
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IL-18 cytokine derived from the enteric nervous system is important for intestinal immunity
Interleukin 18 (IL-18) is a proinflammatory cytokine, which induces cell-mediated immune reaction upon bacterial infection. In the intestine, it is known that IL-18 is produced in immune and…
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Cholesterol Homeostasis Modulates Platinum Sensitivity in Human Ovarian Cancer
Ovarian cancer is one of the most severe types of cancers that women can suffer from during their lifetimes. It is the cancer’s tendency for frequent relapses and drug resistance that leads to…
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Computational Clearing - Enhance 3D Specimen Imaging
This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and improvements within a researcher's imaging-related…
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THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows
This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in retina sections and RNA molecules in cancer tissue…
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Evaluating Axon Regeneration After Brain or Spine Trauma of Mice
Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and…
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Interview with Magali Mondin on THUNDER Imagers
Magali Mondin, an engineer at the BIC (Bordeaux Imaging Center) in France, describes her experience using a THUNDER Imager in this interview. She had the opportunity to test extensively the THUNDER…
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Drosophila Testis Niche Stem Cells – Three Color Computational Clearing
Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only…
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Alzheimer Plaques: fast Visualization in Thick Sections
More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…
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Real time images of 3D specimens with sharp contrast free of haze
THUNDER Imagers deliver in real time images of 3D specimens with sharp contrast, free of the haze or out-of-focus blur typical of widefield systems. They can even image clearly places deep inside a…
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Fields of Application
Pharmaceutical Chemical Research
Your role in pharmaceutical and chemical manufacturing, research and development requires microscope, camera and software solutions to help you clearly and precisely visualize, analyze, and document…
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Live Cell Imaging
Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…
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Fluorescence Microscopy
Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its high sensitivity and high specificity. Fluorescence is a form of luminescence that…
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Zebrafish Research
For the best result during screening, sorting, manipulation, and imaging you need to see details and structures to make the right decisions for your next steps in research.
Known for outstanding…
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Photomanipulation
The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.…
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Neuroscience
Are you working towards a better understanding of neurodegenerative diseases or studying the function of the nervous system? See how you can make breakthroughs with imaging solutions from Leica…
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Organoids and 3D Cell Culture
One of the most exciting recent advancements in life science research is the development of 3D cell culture systems, such as organoids, spheroids, or organ-on-a-chip models. A 3D cell culture is an…
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Virology
Do your research interests focus on viral infection and disease? Find out how you can gain insights into virology with solutions for imaging and sample preparation from Leica Microsystems.
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