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  • Mouse whole-mount retina. Image courtesy of the Experimental Ophthalmology Group, University of Murcia, Spain.

    Fast, High Acuity Imaging and AI-assisted Analysis

    The use of state-of-the-art AI systems is pushing image analysis into a new generation. Challenges like the conflict between imaging power and sample integrity are being overcome with THUNDER’s…
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  • 3D reconstruction of an isolated human islet

    Create New Options for Live Cell Imaging

    The use of state-of-the-art AI systems is pushing image analysis into a new generation. Challenges like the conflict between imaging power and sample integrity are being overcome with THUNDER’s…
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  • Mouse brain showing D2-dopamine receptors

    Expression of D2-Dopamine Receptors in Neurons

    This article discusses how D2-dopamine receptors (D2R) in the substantia-nigra of mouse brain can be more clearly revealed with a THUNDER Imager 3D Assay using Instant Computational Clearing (ICC)…
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  • Images of mouse lung: (left) raw widefield and (right) after the THUNDER technology instant computational clearing (ICC). Courtesy of Dr. Ross Metzger, CA, USA.

    Investigating Pulmonary Vascular Diseases

    This article describes how the cellular and molecular programs governing the formation and maintenance of lung blood vessels, as well as what goes wrong with them when pulmonary vascular diseases…
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  • Epifluorescent and TIRF imaging

    Phosphoprotein Regulates Integrin Endosomal Trafficking

    Integrins are involved in cell-cell and cell-extracellular matrix adhesion. In addition, they mediate several signaling pathways, regulating e.g. cell motility. Caliva et al. discovered, with the help…
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  • Mouse retina_widefield and LVCC

    Visualizing retinal interactions to study eye diseases

    This article shows how interactions between endothelial cells, blood vessels, microglia, and astrocytes in mouse retina can be studied efficiently with a THUNDER Imager 3D Cell Culture and Large…
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  • Whole mouse embryo widefield data and LVCC

    Cranial Nerve Development

    This article demonstrates how fast, high-contrast imaging of mouse embryos with a THUNDER Imager 3D Cell Culture and Large Volume Computational Clearing (LVCC) enable the investigation of axonal…
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  • Blood vessels imaged with THUNDER & Aivia

    Accurately analyze fluorescent widefield images

    The specificity of fluorescence microscopy allows researchers to accurately observe and analyze biological processes and structures quickly and easily, even when using thick or large samples. However,…
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  • Role of Mucins and Glycosylation in Dry Eye Disease

    This article shows how fast, high-contrast, and sharp imaging of stratified human corneal epithelial cells with THUNDER imaging technology for dry eye disease (DED) research allows membrane ridges to…
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  • Images of C2C12 cells

    Skeletal Muscle Adaptation and Fibrotic Diseases

    The mechanisms of how skeletal muscle adapts to fibrotic pathologies can be investigated more efficiently with fast, high-contrast imaging of C2C12 mouse myoblast cells which is described in this…
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  • HeLa Kyoto cells

    How to Improve Live Cell Imaging with Coral Life

    For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…
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  • Optimizing THUNDER Platform for High-Content Slide Scanning

    With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging technology are tested, while user trainability and…
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  • Section of mouse lung

    Monitoring Immunosuppressive Mechanisms from Infection

    This application note discusses the importance of fast, sensitive, and haze-free imaging for the monitoring of immunosuppression in mouse lung epithelial tissue infected with the Puerto Rico 8…
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  • Images of a brain organoid derived from iPSCs acquired with a THUNDER Imager 3D Cell Culture.

    “Brains-In-A-Dish” from Induced Pluripotent Stem Cells (iPSCs)

    This article discusses the benefits of using the THUNDER technology for imaging inside 3D human cortical brain organoids. These organoids are derived from human induced pluripotent stem cells (iPSCs)…
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  • Spheroid shown here as a maximum projection of the raw widefield image data (left) and THUNDER image after Instant Computational Clearing (right).

    Developing Heart Pacemaker Cells from Cardiac Spheroids

    During the last decade, 3D cell culture has been established as a more realistic model compared to classical 2D culture systems. Cells can develop into miniature 3D objects, so called spheroids, which…
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  • Putting Dynamic Live Cell Data into the Ultrastructural Context

    With workflow Coral Life, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the right…
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  • Electroporated nerve cells (green), specific neuronal markers (magenta) and cell nuclei (white), computational cleared.

    Into the Third Dimension with "Wow Effect"- Observe Cells in 3D and Real-Time

    Life is fast, especially for a cell. As a rule, cells should be examined under physiological conditions which are as close as possible to their natural environment. New technologies offer tremendous…
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  • Lung organoid taken at the "liquid-air interface" with a THUNDER Imager 3D Cell Culture

    Observing 3D Cell Cultures During Development

    3D cell cultures, such as organoids and spheroids, give insights into cells and their interactions with their microenvironment. These 3D cell cultures are playing an increasingly important role for…
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  • Cytosolic Ca2+ measurement using ratiometric Fura 2 dye

    Cytosolic Calcium Ions in Melanoma Cancer Cells

    In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging is presented…
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  • Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells.

    Download The Guide to Live Cell Imaging

    In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide range of important considerations to take to…
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  • Studying Cell Division

    Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
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  • Influenza (red) infected primary porcine lung epithelial grown in a multilayer of about 60 µm

    Influenza-host Interactions in 3D Specimens

    Stefan Finke studies virus-host interactions. It turns out that the ways the virus and host interact are different when comparing results from classical laboratory monolayer cell lines and “close to…
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  • Scaffold composed of fluorescent fibers

    Finding new Scaffolds for Tissue Engineering

    Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to creating in vitro disease models. Scaffold architecture…
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  • The Power of Pairing Adaptive Deconvolution with Computational Clearing

    Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with sources of noise. In this technical brief, learn how the…
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  • Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics

    Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell…
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  • Studying Autoimmune Disease

    This article discusses how autoimmune diseases, like systemic lupus erythematosus (SLE), can be studied more efficiently using thick, 3D kidney tissue specimens visualized with a THUNDER Imager . SLE…
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  • From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy

    Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…
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  • Studying Human Brain Development and Disease

    Neural spheroids created from human induced pluripotent stem cells (iPSCs) provide effective and novel tools for studying brain development, as well as the underlying pathological mechanisms of…
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  • An Introduction to Computational Clearing

    Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
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  • Factors to Consider When Selecting a Research Microscope

    An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
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  • Image courtesy of Dr. Stefan Finke, Friedrich-Loeffler- Institute, Riems, Germany.

    How Can Immunofluorescence Aid Virology Research?

    Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…
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  • iPSC cells

    Studying Natural Killer (NK) Cells Derived from Induced Pluripotent Stem Cells (iPSC)

    The study of natural killer (NK) cells holds tremendous promise for developing novel immunotherapies. NK cells derived from induced pluripotent stem cells (iPSCs) can be used to create an easily…
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  • Getting Sharper 3D Images of Thick Biological Specimens with Widefield Microscopy

    Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
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  • IL-18 cytokine derived from the enteric nervous system is important for intestinal immunity

    Interleukin 18 (IL-18) is a proinflammatory cytokine, which induces cell-mediated immune reaction upon bacterial infection. In the intestine, it is known that IL-18 is produced in immune and…
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  • Cholesterol Homeostasis Modulates Platinum Sensitivity in Human Ovarian Cancer

    Ovarian cancer is one of the most severe types of cancers that women can suffer from during their lifetimes. It is the cancer’s tendency for frequent relapses and drug resistance that leads to…
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  • Computational Clearing - Enhance 3D Specimen Imaging

    This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and improvements within a researcher's imaging-related…
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  • THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows

    This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in retina sections and RNA molecules in cancer tissue…
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  • Evaluating Axon Regeneration After Brain or Spine Trauma of Mice

    Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and…
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  • Interview with Magali Mondin on THUNDER Imagers

    Magali Mondin, an engineer at the BIC (Bordeaux Imaging Center) in France, describes her experience using a THUNDER Imager in this interview. She had the opportunity to test extensively the THUNDER…
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  • Drosophila Testis Niche Stem Cells – Three Color Computational Clearing

    Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only…
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  • Alzheimer Plaques: fast Visualization in Thick Sections

    More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…
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  • Real time images of 3D specimens with sharp contrast free of haze

    THUNDER Imagers deliver in real time images of 3D specimens with sharp contrast, free of the haze or out-of-focus blur typical of widefield systems. They can even image clearly places deep inside a…
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  • Fields of Application

  • Pharmaceutical Chemical Research

    Your role in pharmaceutical and chemical manufacturing, research and development requires microscope, camera and software solutions to help you clearly and precisely visualize, analyze, and document…
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  • This confocal image shows one scan layer of the acoel worm Isodiametra pulchra. The following parts are stained: cyan: nuclei, green: stem cells, magenta: expression of the genes T-Brain/Eomes (gonads und oocytes). Left: anterior. The worm is about 1 mm of size. Photo: Aina Børve, Sars

    Live Cell Imaging

    Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…
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  • Fluorescence Microscopy

    Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its high sensitivity and high specificity. Fluorescence is a form of luminescence that…
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  • Zebrafish Research

    For the best result during screening, sorting, manipulation, and imaging you need to see details and structures to make the right decisions for your next steps in research. Known for outstanding…
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  • Leica photomanipulation

    Photomanipulation

    The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.…
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  • Neuroscience

    Are you working towards a better understanding of neurodegenerative diseases or studying the function of the nervous system? See how you can make breakthroughs with imaging solutions from Leica…
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  • Organoids and 3D Cell Culture

    One of the most exciting recent advancements in life science research is the development of 3D cell culture systems, such as organoids, spheroids, or organ-on-a-chip models. A 3D cell culture is an…
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  • Virology

    Do your research interests focus on viral infection and disease? Find out how you can gain insights into virology with solutions for imaging and sample preparation from Leica Microsystems.
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