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  • Pharmaceutical Chemical Engineering Research

    Your role in pharmaceutical and chemical manufacturing, research and development requires microscope, camera and software solutions to help you clearly and precisely visualize, analyze, and document…
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  • Live Cell Imaging

    Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…
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  • Fluorescence Microscopy

    Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its high sensitivity and high specificity. Fluorescence is a form of luminescence that…
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  • Zebrafish Research

    For the best result during screening, sorting, manipulation, and imaging you need to see details and structures to make the right decisions for your next steps in research. Known for outstanding…
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  • Leica photomanipulation

    Photomanipulation

    The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.…
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  • Neuroscience

    Are you working towards a better understanding of neurodegenerative diseases or studying the function of the nervous system? See how you can make breakthroughs with imaging solutions from Leica…
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  • Organoids and 3D Cell Culture

    One of the most exciting recent advancements in life science research is the development of 3D cell culture systems, such as organoids, spheroids, or organ-on-a-chip models. A 3D cell culture is an…
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  • Virology

    Do your research interests focus on viral infection and disease? Find out how you can gain insights into virology with solutions for imaging and sample preparation from Leica Microsystems.
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  • Science Lab Topic: Fluorescence Microscopy

    Optimizing THUNDER Platform for High-Content Slide Scanning

    With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging technology are tested, while user trainability and…
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  • Section of mouse lung
    Science Lab Topic: Fluorescence Microscopy

    Monitoring Immunosuppressive Mechanisms from Infection

    This application note discusses the importance of fast, sensitive, and haze-free imaging for the monitoring of immunosuppression in mouse lung epithelial tissue infected with the Puerto Rico 8…
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  • Images of a brain organoid derived from iPSCs acquired with a THUNDER Imager 3D Cell Culture.
    Science Lab Topic: Fluorescence Microscopy

    “Brains-In-A-Dish” from Induced Pluripotent Stem Cells (iPSCs)

    This article discusses the benefits of using the THUNDER technology for imaging inside 3D human cortical brain organoids. These organoids are derived from human induced pluripotent stem cells (iPSCs)…
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  • Spheroid shown here as a maximum projection of the raw widefield image data (left) and THUNDER image after Instant Computational Clearing (right).
    Science Lab Topic: Widefield Microscopy

    Developing Heart Pacemaker Cells from Cardiac Spheroids

    During the last decade, 3D cell culture has been established as a more realistic model compared to classical 2D culture systems. Cells can develop into miniature 3D objects, so called spheroids, which…
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  • Science Lab Topic: EM Sample Preparation

    Putting Dynamic Live Cell Data into the Ultrastructural Context

    With the Leica Nano Workflow, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…
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  • Electroporated nerve cells (green), specific neuronal markers (magenta) and cell nuclei (white), computational cleared.
    Science Lab Topic: Widefield Microscopy

    Into the Third Dimension with "Wow Effect"- Observe Cells in 3D and Real-Time

    Life is fast, especially for a cell. As a rule, cells should be examined under physiological conditions which are as close as possible to their natural environment. New technologies offer tremendous…
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  • Lung organoid taken at the "liquid-air interface" with a THUNDER Imager 3D Cell Culture
    Science Lab Topic: Widefield Microscopy

    Observing 3D Cell Cultures During Development

    3D cell cultures, such as organoids and spheroids, give insights into cells and their interactions with their microenvironment. These 3D cell cultures are playing an increasingly important role for…
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  • Cytosolic Ca2+ measurement using ratiometric Fura 2 dye
    Science Lab Topic: Widefield Microscopy

    Cytosolic Calcium Ions in Melanoma Cancer Cells

    In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging is presented…
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  • Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells.
    Science Lab Topic: Widefield Microscopy

    Download The Guide to Live Cell Imaging

    In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide range of important considerations to take to…
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  • Science Lab Topic: Fluorescence Microscopy

    Studying Cell Division

    Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
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  • Influenza (red) infected primary porcine lung epithelial grown in a multilayer of about 60 µm
    Science Lab Topic: Fluorescence Microscopy

    Viral Infections – Studying Influenza-host Interactions in 3D Specimens

    Stefan Finke studies virus-host interactions. It turns out that the ways the virus and host interact are different when comparing results from classical laboratory monolayer cell lines and “close to…
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  • Scaffold composed of fluorescent fibers
    Science Lab Topic: Fluorescence Microscopy

    Finding new Scaffolds for Tissue Engineering

    Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to creating in vitro disease models. Scaffold architecture…
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  • Science Lab Topic: Widefield Microscopy

    The Power of Pairing Adaptive Deconvolution with Computational Clearing

    Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with sources of noise. In this technical brief, learn how the…
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  • Science Lab Topic: Fluorescence Microscopy

    Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics

    Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell…
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  • Science Lab Topic: Fluorescence Microscopy

    Studying Autoimmune Disease

    This article discusses how autoimmune diseases, like systemic lupus erythematosus (SLE), can be studied more efficiently using thick, 3D kidney tissue specimens visualized with a THUNDER Imager . SLE…
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  • Science Lab Topic: Fluorescence Microscopy

    From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy

    Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…
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  • Science Lab Topic: Widefield Microscopy

    Studying Human Brain Development and Disease

    Neural spheroids created from human induced pluripotent stem cells (iPSCs) provide effective and novel tools for studying brain development, as well as the underlying pathological mechanisms of…
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  • Science Lab Topic: Widefield Microscopy

    An Introduction to Computational Clearing

    Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
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  • Science Lab Topic: Basics in Microscopy

    Factors to Consider When Selecting a Research Microscope

    An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
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  • Image courtesy of Dr. Stefan Finke, Friedrich-Loeffler- Institute, Riems, Germany.
    Science Lab Topic: Confocal Microscopy

    How Can Immunofluorescence Aid Virology Research?

    Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…
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  • iPSC cells
    Science Lab Topic: Widefield Microscopy

    Studying Natural Killer (NK) Cells Derived from Induced Pluripotent Stem Cells (iPSC)

    The study of natural killer (NK) cells holds tremendous promise for developing novel immunotherapies. NK cells derived from induced pluripotent stem cells (iPSCs) can be used to create an easily…
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  • Science Lab Topic: Basics in Microscopy

    Getting Sharper 3D Images of Thick Biological Specimens with Widefield Microscopy

    Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
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  • Science Lab Topic: Fluorescence Microscopy

    IL-18 cytokine derived from the enteric nervous system is important for intestinal immunity

    Interleukin 18 (IL-18) is a proinflammatory cytokine, which induces cell-mediated immune reaction upon bacterial infection. In the intestine, it is known that IL-18 is produced in immune and…
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  • Science Lab Topic: Fluorescence Microscopy

    Cholesterol Homeostasis Modulates Platinum Sensitivity in Human Ovarian Cancer

    Ovarian cancer is one of the most severe types of cancers that women can suffer from during their lifetimes. It is the cancer’s tendency for frequent relapses and drug resistance that leads to…
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  • Science Lab Topic: Widefield Microscopy

    Computational Clearing - Enhance 3D Specimen Imaging

    This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and improvements within a researcher's imaging-related…
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  • Science Lab Topic: Widefield Microscopy

    THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows

    This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in retina sections and RNA molecules in cancer tissue…
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  • Science Lab Topic: Widefield Microscopy

    Evaluating Axon Regeneration After Brain or Spine Trauma of Mice

    Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and…
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  • Science Lab Topic: Fluorescence Microscopy

    Interview with Magali Mondin on THUNDER Imagers

    Magali Mondin, an engineer at the BIC (Bordeaux Imaging Center) in France, describes her experience using a THUNDER Imager in this interview. She had the opportunity to test extensively the THUNDER…
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  • Science Lab Topic: Widefield Microscopy

    Drosophila Testis Niche Stem Cells – Three Color Computational Clearing

    Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only…
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  • Science Lab Topic: Widefield Microscopy

    Alzheimer Plaques: fast Visualization in Thick Sections

    More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…
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  • Science Lab Topic: Widefield Microscopy

    THUNDER Technology Note

    So far, widefield microscopy was not suitable to image larger volumes, since the contrast of the recorded fluorescence image is reduced by the background (BG) mainly originating from out of focus…
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