Industrial Microscopy

Dive deep into detailed articles and webinars focusing on efficient inspection, optimized workflows, and ergonomic comfort in industrial and pathological contexts. Topics covered include quality control, materials analysis, microscopy in pathology, among many others. This is the place where you get valuable insights into using cutting-edge technologies for improving precision and efficiency in manufacturing processes as well as accurate pathological diagnosis and research.

Immersion Freezing for Cryo-Transmission Electron Microscopy: Fundamentals

The high vacuum required in a transmission electron microscope (TEM) greatly impairs the ability to study specimens naturally occurring in an aqueous phase: exposing "wet" specimens to a pressure…

Video Tutorials: Filling and Assembling of Different Carriers for High-Pressure Freezing

High pressure freezing (HPF) is a cryo-fixation method primarily for biological samples, but also for a variety of non-biological materials. It is a technique that yields optimal preservation in many…

Brief Introduction to Specimen Trimming

Before ultrathin sectioning a sample with an ultramicrotome it has to be pre-prepared. For this pre-preparation, special attention must be paid to the sample size (size of the section), location of…

Glycerol Spraying/Platinum Low Angle Rotary Shadowing of DNA with the Leica EM ACE600 e-beam

Glycerol spraying/low angle rotary shadowing (Aebi and Baschong, 2006) is a preparation technique used in biology to visualize structures yielding insufficent contrast with other techniques, due to…

Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips

The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as…

Brief Introduction to Freeze Substitution

Freeze-substitution is a process of dehydration, performed at temperatures low enough to avoid the formation of ice crystals and to circumvent the damaging effects observed after ambient-temperature…

Brief Introduction to Contrasting for EM Sample Preparation

Since the contrast in the electron microscope depends primarily on the differences in the electron density of the organic molecules in the cell, the efficiency of a stain is determined by the atomic…

Brief Introduction to Glass Knifemaking for Electron and Light Microscope Applications

Glass knives are used in an ultramicrotome to cut ultrathin slices of samples for electron and light microscope applications. For resin and for cryo sections (Tokuyasu samples) the knife edge must be…

Perusing Alternatives for Automated Staining of TEM Thin Sections

Contrast in transmission electron microscopy (TEM) is mainly produced by electron scattering at the specimen: Structures that strongly scatter electrons are referred to as electron dense and appear as…