Science Lab
Science Lab
Bienvenido al portal de conocimiento de Leica Microsystems. Aquí encontrará investigación científica y material didáctico sobre el tema de la microscopía. El portal ayuda a principiantes, profesionales experimentados y científicos por igual en su trabajo diario y en sus experimentos. Explore tutoriales interactivos y notas de aplicación, descubra los fundamentos de la microscopía, así como las tecnologías de gama alta. Forme parte de la comunidad Science Lab y comparta sus conocimientos.
Filter articles
Tags
Story Type
Products
Loading...
Bacteria Protocol - Critical Point Drying of E. coli for SEM
Application Note for Leica EM CPD300 - Critical point drying of E. coli with subsequent platinum / palladium coating and SEM analysis. Sample was inserted into a filter disc (Pore size: 16 - 40 μm)…
Loading...
Epoxy Resin Embedding of Animal and Human Tissues for Pathological Diagnosis and Research
Application Note for Leica EM AMW - The tissues were fixed in the modified Karnovsky fixative generally by immersion overnight (at minimum 4h at room temperature). Then pieces of approx. 1mm3 were cut…
Loading...
Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast
Application Note for Leica EM HPM100 - The sections are of yeast frozen with a Leica HPM100 high pressure freezer in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer…
Loading...
Human Blood Cells Protocol
Application Note for Leica EM CPD300 - Life Science Research. Species: Human (Homo sapiens)
Critical point drying of human blood with subsequent platinum / palladium coating and SEM analysis.
Loading...
Improvement of Metallic Thin Films for HR-SEM by Using DC Magnetron Sputter Coater
Preparation techniques, like several kinds of coating methods play an important role for high resolution scanning electron microscopy (HR-SEM). Nonconductive sample like biological and synthetic…
Loading...
Immersion Freezing for Cryo-Transmission Electron Microscopy: Applications
A well established usage case for cryo-TEM is three-dimensional reconstruction of isolated macromolecules, virus particles, or filaments. On one hand, these approaches are based on averaging of…
Loading...
Immersion Freezing for Cryo-Transmission Electron Microscopy: Fundamentals
The high vacuum required in a transmission electron microscope (TEM) greatly impairs the ability to study specimens naturally occurring in an aqueous phase: exposing "wet" specimens to a pressure…
Loading...
Video Tutorials: Filling and Assembling of Different Carriers for High-Pressure Freezing
High pressure freezing (HPF) is a cryo-fixation method primarily for biological samples, but also for a variety of non-biological materials. It is a technique that yields optimal preservation in many…
Loading...
Brief Introduction to Freeze Fracture and Etching
Freeze fracture describes the technique of breaking a frozen specimen to reveal internal structures. Freeze etching is the sublimation of surface ice under vacuum to reveal details of the fractured…
