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Science Lab

Science Lab

Bienvenue sur le portail de connaissances de Leica Microsystems. Vous y trouverez des recherches scientifiques et du matériel didactique sur le thème de la microscopie. Le portail aide les débutants, les praticiens expérimentés et les scientifiques dans leur travail quotidien et leurs expériences. Explorez les didacticiels interactifs et les notes d'application, découvrez les bases de la microscopie ainsi que les technologies de pointe. Faites partie de la communauté Science Lab et partagez votre expertise.

Bacteria Protocol - Critical Point Drying of E. coli for SEM

Application Note for Leica EM CPD300 - Critical point drying of E. coli with subsequent platinum / palladium coating and SEM analysis. Sample was inserted into a filter disc (Pore size: 16 - 40 μm)…

Mouse liver (immersion fixed)

Epoxy Resin Embedding of Animal and Human Tissues for Pathological Diagnosis and Research

Application Note for Leica EM AMW - The tissues were fixed in the modified Karnovsky fixative generally by immersion overnight (at minimum 4h at room temperature). Then pieces of approx. 1mm3 were cut…

Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast

Application Note for Leica EM HPM100 - The sections are of yeast frozen with a Leica HPM100 high pressure freezer in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer…

Human Blood Cells Protocol

Application Note for Leica EM CPD300 - Life Science Research. Species: Human (Homo sapiens) Critical point drying of human blood with subsequent platinum / palladium coating and SEM analysis.

Improvement of Metallic Thin Films for HR-SEM by Using DC Magnetron Sputter Coater

Preparation techniques, like several kinds of coating methods play an important role for high resolution scanning electron microscopy (HR-SEM). Nonconductive sample like biological and synthetic…

Immersion Freezing for Cryo-Transmission Electron Microscopy: Applications

A well established usage case for cryo-TEM is three-dimensional reconstruction of isolated macromolecules, virus particles, or filaments. On one hand, these approaches are based on averaging of…

Immersion Freezing for Cryo-Transmission Electron Microscopy: Fundamentals

The high vacuum required in a transmission electron microscope (TEM) greatly impairs the ability to study specimens naturally occurring in an aqueous phase: exposing "wet" specimens to a pressure…

Video Tutorials: Filling and Assembling of Different Carriers for High-Pressure Freezing

High pressure freezing (HPF) is a cryo-fixation method primarily for biological samples, but also for a variety of non-biological materials. It is a technique that yields optimal preservation in many…

Brief Introduction to Freeze Fracture and Etching

Freeze fracture describes the technique of breaking a frozen specimen to reveal internal structures. Freeze etching is the sublimation of surface ice under vacuum to reveal details of the fractured…