Life Science Research

This is the place to expand your knowledge, research capabilities, and practical applications of microscopy in various scientific fields. Learn how to achieve precise visualization, image interpretation, and research advancements. Find insightful information on advanced microscopy, imaging techniques, sample preparation, and image analysis. Topics covered include cell biology, neuroscience, and cancer research with a focus on cutting-edge applications and innovations.

U2OS cells transfected with an Mx1-GFP plasmid (signal enhanced using Alexa Fluor 488-conjugared anti-GFP antibody) and co-stained for nuclear DNA (Hoechst 33342), microtubules (Alexa 555) and F-actin (ATTO 643). Image was captured on Mateo FL.

Microscopy and AI Solutions for 2D Cell Culture

This eBook explores the integration of microscopy and AI technologies in 2D cell culture workflows. It highlights how traditional imaging methods—such as brightfield, phase contrast, and…

Artificial Intelligence (AI) segmentation used in conjunction with LMD to increase discovery throughput.

Biomarker Discovery with Laser Microdissection

Explore the potential of spatial proteomics workflows, such as Deep Visual Proteomics (DVP), to decipher pathology mechanisms and uncover druggable targets. Altered protein expression, abundance, or…

Digital microscopy simplifies documenting cell-culture results electronically while following 21 CFR part 11 guidelines for biopharma.

Introduction to 21 CFR Part 11 for Electronic Records of Cell Culture

This article provides an introduction to the recommendations of 21 CFR Part 11 from the FDA, specifically focusing on the audit trail and user management in the context of cell-culture laboratories.…

Molecular structure of the green fluorescent protein (GFP)

Introduction to Fluorescent Proteins

Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.

C. elegans adult hermaphrodite gonades acquired using THUNDER Imager. Staining: blue - DAPI (nucleus), green - SP56 (sperm), red - RME-2 (oocyte), magenta - PGL-1 (RNA + protein granules). Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany.

Life Science Research: Which Microscope Camera is Right for You?

Deciding which microscope camera best fits your experimental needs can be daunting. This guide presents the key factors to consider when selecting the right camera for your life science research.

Multi-tissue array with 4 markers shown including DAPI, NaKATPase, PanCk, and Vimentin.

Spatial Biology: Learning the Landscape

Spatial Biology: Understanding the organization and interaction of molecules, cells, and tissues in their native spatial context

Fluorescence microscope image of a life-science specimen

An Introduction to Fluorescence

This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.

Pancreatic Ductal Adenocarcinoma with 5 biomarkers shown – SMA, PanCK PCK26, PanCK AE1, Vimentin, and Glut1.

Dig Deeper Into the Complexities of Pancreatic Cancer with Multiplex Imaging

Cell DIVE is an iterative staining workflow for multiplexed imaging that unveils biological pathways to dig deeper into the complexities of pancreatic cancer.

Complex Made Simple: Antibodies in Multiplexed Imaging

Build panels, plan studies, and get the most from precious reagents using this antibody multiplexing guide from Leica Microsystems