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Brief Introduction to Coating Technology for Electron Microscopy
Coating of samples is required in the field of electron microscopy to enable or improve the imaging of samples. Creating a conductive layer of metal on the sample inhibits charging, reduces thermal…
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Carbon Thickness Evaluation in Electron Microscopy
The coating layers applied and used for electron microscopy imaging are commonly controlled and measured by quartz crystals. These crystals oscillate with a certain frequency (around 6 megahertz when…
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Brief Introduction to High-Pressure Freezing
Water is the most abundant cellular constituent and therefore important for preserving cellular ultra-structure. Currently the only way to fix cellular constituents without introducing significant…
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Perusing Alternatives for Automated Staining of TEM Thin Sections
Contrast in transmission electron microscopy (TEM) is mainly produced by electron scattering at the specimen: Structures that strongly scatter electrons are referred to as electron dense and appear as…
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Brief Introduction to Critical Point Drying
One of the uses of the Scanning Electron Microscope (SEM) is in the study of surface morphology in biological applications which requires the preservation of the surface details of a specimen. Samples…