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How to Prepare your Specimen for Immunofluorescence Microscopy
Immunofluorescence (IF) specimen preparations can be analyzed by various fluorescence microscopy techniques, depending on the research application. IF is indispensable for researchers using a simple…
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Live-Cell Imaging Techniques
The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focused on dynamic…
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Applying AI and Machine Learning in Microscopy and Image Analysis
Prof. Emma Lundberg is a professor in cell biology proteomics at KTH Royal Institute of Technology, Sweden. She is also the director of the Cell Atlas, an integral part of the Swedish-based Human…
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Using Machine Learning in Microscopy Image Analysis
Recent exciting advances in microscopy technologies have led to exponential growth in quality and quantity of image data captured in biomedical research. However, analyzing large and increasingly…
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The AI-Powered Pixel Classifier
Achieving reproducible results manually requires expertise and is tedious work. But now there is a way to overcome these challenges by speeding up this analysis to extract the real value of the image…
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Multicolor Microscopy: The Importance of Multiplexing
The term multiplexing refers to the use of multiple fluorescent dyes to examine various elements within a sample. Multiplexing allows related components and processes to be observed in parallel,…
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Considerations for Multiplex Live Cell Imaging
Simultaneous multicolor imaging for successful experiments: Live-cell imaging experiments are key to understand dynamic processes. They allow us to visually record cells in their living state, without…
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A New Method for Convenient and Efficient Multicolor Imaging
The technique combining hyperspectral unmixing and phasor analysis was developed to simplify the process of getting images from a sample labeled with multiple fluorophores. This aggregate method…
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Imaging of Anti-Cancer Drug Uptake in Spheroids using DLS
Spheroid 3D cell culture models mimic the physiology and functions of living tissues making them a useful tool to study tumor morphology and screen anti-cancer drugs. The drug AZD2014 is a recognized…