


The Microscopy Knowledge Portal
The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
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Topics – A Vast Amount of Expert Knowledge
- Basics in Microscopy
- Confocal Microscopy
- Digital Microscopy
- Dentistry
- EM Sample Preparation
- Fluorescence Microscopy
- Light Sheet Microscopy
- Neurosurgery
- Optical Coherence Tomography
- Super-Resolution
- Stereo Microscopy
- Surface Metrology
- Quality Assurance
- Otolaryngology (ENT)
- Ophthalmology
- Plastic Reconstructive
- Widefield Microscopy
- More
Latest Articles
Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…Read articleKeeping Particulate Contamination Under Control in Pharmaceutical Products
This article describes how a 2-methods-in-1 solution combining optical microscopy and laser induced breakdown spectroscopy (LIBS) can be utilized for identification of particulate contaminants in the…Read articleWriting the NIH Research (R01) grant: Strategies for Success
With highly competitive NIH budgets and ever-increasing restrictions on federal spending, getting noticed and winning an NIH R grant is far more difficult and highly competitive than it has ever been.…Read articlePursuing private foundation and corporate grant opportunities in Life Science Research
Devon Brewer describes the essentials for pursuing research grants from private foundations and corporations.Read articleHow FLIM Microscopy helps to detect microplastic pollution
The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…Read articleHigh-pressure freezing: Revealing functional mechanisms of synaptic transmission
Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…Read articleFast Visual and Chemical Analysis of Contamination and Underlying Layers
Visual and chemical analysis of contamination on materials with a 2-methods-in-1 solution leading to an efficient, more complete analysis workflow is described in this report. A 2-in-1 solution,…Read articleA New World of Confocal Applications with the Next Generation White Light Lasers
As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is…Read articleHand-Held OCT Clinical & Research Applications
Hand-held Optical Coherence Tomography (OCT) has revolutionized pediatric ophthalmology and has had a significant impact on ophthalmology in general. The Leica Envisu C2300 hand-held OCT allows to…Read articleFrom Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…Read articleImmediate Breast Reconstruction: Benefits of GLOW800 Augmented Reality
Dr. Harold Chatel is a Plastic and Reconstructive Surgeon in a leading hospital in Paris, France. One of his areas of expertise is oncological reconstructive surgery and immediate breast…Read articleOncological Reconstructive Surgery with the Leica M530 OHX Microscope
Precision is essential in oncological reconstructive surgery, in particular when it relies on free flap techniques. Microsurgical microscopes provide optimal visualization and help streamline the…Read articleOncological Reconstructive Surgery: Why Use a Microscope
Recent advances in microsurgery are enhancing breast reconstruction for oncology patients, allowing both functional and aesthetic rehabilitation. More and more surgeons are adopting surgical…Read articleOncological Reconstructive Surgery: Benefits of Microscope & Integrated Vascular Fluorescence Use
Oncological reconstructive surgery requires high precision and attention to details. It can pose a number of challenges: long operating time, flap necrosis and other complications. Leica visualization…Read articleHow does an Automated Rating Solution for Steel Inclusions Work?
The rating of non-metallic inclusions (NMIs) to determine steel quality is critical for many industrial applications. For an efficient and cost-effective steel quality evaluation, an automated NMI…Read article