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Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins
We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian…
Jun 04, 2015Abstract -
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Electron Tomography of Cryo-Immobilized Plant Tissue: A Novel Approach to Studying 3D Macromolecular Architecture of Mature Plant Cell Walls In Situ
Cost-effective production of lignocellulosic biofuel requires efficient breakdown of cell walls present in plant biomass to retrieve the wall…
Nov 17, 2014Abstract -
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Capturing Cellular Dynamics with Millisecond Temporal Resolution
The combination of two powerful techniques: optogenetics and high-pressure freezing now makes it possible to visualize a dynamic cellular activity…
May 12, 2014Story -
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Freeze Substitution of Trypanosoma brucei
Chemical fixation of biological specimens for ultrastructural investigation is a relatively slow and selective process, and therefore a common source…
Mar 18, 2014Story -
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Brief Introduction to Freeze Substitution
Freeze-substitution is a process of dehydration, performed at temperatures low enough to avoid the formation of ice crystals and to circumvent the…
Feb 04, 2014Tutorial -
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From Dynamic Live Cell Imaging to 3D Ultrastructure
To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines…
Aug 28, 2013Abstract