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Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on…
Sep 24, 2020Story - Loading...
Eyepieces, Objectives and Optical Aberrations
For most microscope applications, there are generally only two sets of optics which are adjusted by the user, namely, the objectives and the…
Aug 28, 2017Story - Loading...
Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps
The technique of Koehler Illumination is one of the most important and fundamental techniques in achieving optimum imaging in any given light…
Aug 17, 2017Story - Loading...
Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution
To examine specimens at high magnifications using the microscope, there are a number of factors which need to be taken into consideration. These…
Aug 09, 2017Story - Loading...
Collecting Light: The Importance of Numerical Aperture in Microscopy
Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish detail in a specimen viewed down the microscope. NA…
Jul 12, 2017Story - Loading...
Optimization of the Interplay of Optical Components for Aberration free Microscopy
Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to…
May 31, 2017Story - Loading...
Microscope Resolution: Concepts, Factors and Calculation
In microscopy, the term ‘resolution’ is used to describe the ability of a microscope to distinguish detail. In other words, this is the minimum…
Dec 02, 2016Story - Loading...
BABB Clearing and Imaging for High Resolution Confocal Microscopy
Multipohoton microscopy experiment using Leica TCS SP8 MP and Leica 20x/0.95 NA BABB immersion objective. Understanding kidney microanatomy is key…
Sep 13, 2016Story - Loading...
Infinity Optical Systems
“Infinity Optics” refers to the concept of a beam path with parallel rays between the objective and the tube lens of a microscope. Flat optical…
Mar 11, 2016Story - Loading...
How to Clean Microscope Optics
Clean microscope optics are essential for obtaining good microscope images. If they are dirty, the microscope should be cleaned to avoid a loss of…
Jul 08, 2012Tutorial - Loading...
Optical Microscopes – Some Basics
The optical microscope has been a standard tool in life science as well as material science for more than one and a half centuries now. To use this…
May 02, 2012Tutorial - Loading...
Beware of "Empty" Magnification
This article explains how to avoid the phenomen of "empty magnification" in microscopy.
May 18, 2008Tutorial