Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Projection of a confocal z-stack before and after LIGHTNING applied. Sum159 cells, human breast cancer cells kindly provided by Ievgeniia Zagoriy, Mahamid Group, EMBL Heidelberg, Germany. Blue – Hoechst - indicates nuclei, Green – MitoTracker Green – mitochondria, and red – Bodipy - lipid droplets.

New Imaging Tools for Cryo-Light Microscopy

LIGHTNING super-resolution detection concept and TauSense technology facilitate better cryogenic fluorescence imaging for advanced cryo-correlative workflows. The quality of fluorescence microscope…

Correlation of markers in the LM and the FIB image.

How to Target Fluorescent Structures in 3D for Cryo-FIB Milling

This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…

LNG-non-LNGHeLa cells labeled with light blue –Hoechst, Nuclei

Precise 3D Targeting for EM Imaging - Access What Matters

Find out how the seamless cryo-electron tomography workflow Coral Cryo uses confocal super resolution to target your structure of interest more precisely.