3D Imaging
RSS feed
Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points
Cancer research unsing multiphoton microscopy and 3D electron microscopy. Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient…Read article
Third Harmonic Generation Microscopy – Label-Free 3D-Tissue Imaging and Blood Flow Characterization
THG microscopy as special variants of multiphoton microscopy. Third Harmonic Generation (THG) microscopy is a non-fluorescent multi-photon technique that combines the advantages of label-free…Read article
Nanoscale or Microscale Structures Formed in Polymers Containing Nanotubes Greatly Enhance the Electrical Conductivity: Potential Applications for Photovoltaic Devices
The excellent mechanical and electrical properties of carbon nanotubes have led to them being exploited for the creation of a new class of high performance polymer composites. Due to important…Read article
Nature Methods: Light-sheet Fluorescence Microscopy - Method of the Year 2014
Just about everyone who has examined fluorescent samples under the microscope is aware of the constant struggle to have enough signal to see the labeled structures while also avoiding fluorophore…Read article
3D STED (Stimulated Emission Depletion) Microscopy
The resolution needed to image subcellular architecture and dynamics in light microscopy is hindered by the diffraction limits as described by Ernst Abbe. Simply stated, structures smaller than 200…Read article
Video Interview with Timo Zimmermann
The first super-resolution image he saw was an eye opener for him: "It was not just structures that got smaller. I was looking at a sample that I specifically had high hopes of seeing another layer of…Read article
Are Your Digital Microscope Measurements Accurate and Reliable?
For certain applications, such as the production and maintenance of automobiles, aircraft, or power plants, quality control and reliability assurance, as well as safety and health inspection, accurate…Read article
Laser Microdissection (LMD) and Fancy Applications
New and far-reaching applications have recently been opened up in the field of laser microdissection. In addition to conventional dissection, the laser microdissection system (LMD) of Leica…Read article
Three-Dimensional Super-Resolution GSDIM Microscopy
With the new 3D GSDIM technique structures like the Golgi and the microtubular network are resolved not only laterally, but also in a third dimension. The principle is based on the use of optical…Read article
From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy
To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines confocal time-lapse images of GFP-tagged proteins…Read article
Light Sheet Fluorescence Microscopy: Beyond the Flatlands
Light Sheet Fluorescence Microscopy (LISH-M) is a true fluorescence optical sectioning technique, first described by Heinrich Siedentopf in 1902 under the name of Ultramicroscopy. Light sheet…Read article
Digital Microscopy
Digital microscopy offers clear advantages for a large number of industrial quality inspections, particularly for surface analysis. Here, you can find some videos that show examples of application for…Read article
Developments in Multiphoton Excitation Microscopy
Basics, history, and applications of multiphoton microscopy. Honouring Goeppert-Mayer’s prediction of simultaneous two-photon absorption by an atom or molecule reported in the 1930s in her PhD…Read article
3D Visualization of Surface Structures
One of the main features of a digital microscope is the speed and ease with which it enables surface models to be created of macroscopic and microscopic structures. In a qualitative evaluation, these…Read article
Trends in Microscopy
There are digital cameras, digital TV sets, digital picture frames, digital schools on the internet. Cryptologists design digital signatures, communication researchers speak of digital identity.…Read article